[健康献血者和血栓形成患者中活化蛋白C抵抗（APC-R）及凝血因子V莱顿突变的研究]

[Study of activated protein C resistance(APC-R) and FV Leiden in healthy blood donors and patients with thrombosis].

作者信息

Hong M, Wei W, Song S

机构信息

Department of Hematology, Union Hospital, Tongji Medical University, Wuhan 430022.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 1998 Sep;19(9):455-7.

PMID:11189481

Abstract

OBJECTIVE

To study activated protein C resistance (APC-R) and FV Leiden in Chinese and their role in the pathogenesis of thrombophilia.

METHODS

APC-R was detected by activated partial thromboplastin time (APTT) in the presence and absence of APC. FV Leiden mutation was detected by PCR amplification with sequence-specific primers and restriction enzyme digestion. The subjects included 32 healthy blood donors (control), 38 patients with thrombosis in Wuhan, China, 106 patients with thrombosis in Homburg, Germany. FV Leiden mutation was screened in 12 patients in Wuhan and 106 in Homburg.

RESULTS

There was no significant difference for APC-R between healthy controls and the patients in Wuhan. No FV Leiden mutation was found in 12 patients in Wuhan. The APC-R frequency of the patients in Homburg was significantly higher than those in Wuhan (22.6% and 7.9%, respectively). Eleven patients in Hombery had FV Leiden mutation.

CONCLUSION

APC-R and FV Leiden mutation varied in different regions and races. FV Leiden may not be the main cause of thrombophilia in Chinese.

摘要

目的

研究中国人活化蛋白C抵抗（APC-R）及凝血因子V莱顿突变（FV Leiden）情况及其在血栓形成倾向发病机制中的作用。

方法

采用活化部分凝血活酶时间（APTT）法在有无活化蛋白C（APC）存在的情况下检测APC-R。采用序列特异性引物PCR扩增及限制性内切酶消化法检测FV Leiden突变。研究对象包括32名健康献血者（对照组）、38名中国武汉血栓形成患者、106名德国洪堡血栓形成患者。对武汉的12名患者及洪堡的106名患者进行FV Leiden突变筛查。