Ge T M, Zhang R D, Qin F L, Yu Y J, Xie Y F
Department of Horticulture, Hubei Institute for Nationalities, Enshi 445000.
Sheng Wu Gong Cheng Xue Bao. 2000 Sep;16(5):609-13.
Friable embryogenic calli were obtained on a modified N6 medium (NBD medium) from a winter wheat cultivar "Jinghua No. 1" (Triticum aestivum L. cv. Jinghua No. 1) and were transferred to a modified MS liquid medium (MSDL medium) to initiate embryogenic suspension cultures. Protoplasts were isolated from the suspensions and cultured on a modified MS medium (MSDP medium). The somatic embryoids were formed directly from the protoplasts and germinated into entire plants. The development of the somatic embryoids was very similar to that of zygotic embryos of wheat.
在改良的N6培养基（NBD培养基）上，从冬小麦品种“京花1号”（Triticum aestivum L. cv. Jinghua No. 1）获得了易碎的胚性愈伤组织，并将其转移到改良的MS液体培养基（MSDL培养基）中以启动胚性悬浮培养。从悬浮液中分离出原生质体，并在改良的MS培养基（MSDP培养基）上进行培养。体细胞胚直接从原生质体形成，并萌发成完整植株。体细胞胚的发育与小麦合子胚的发育非常相似。
Zotero 插件