Zhang H, Wu Q, Liu D
Cytogenetics Institute, Nanjing Agricultural University, China.
Chin J Biotechnol. 1995;11(3):207-11.
From the suspension cultures of Gynostemma pentaphyllum (Thumb) Mak. protoplasts were isolated and cultured in a different medium with liquid and nurse culture method. Cell division was observed within 4 days and microcalli were formed within 4 weeks. On an MS solid medium with 1 mg/L of KT and 0.5 mg/L of IAA, protoplast-derived calli differentiated into embryos. Stems and leaves were formed on the MS medium with 1 mg/L of 6-BA and 0.5 mg/L of IAA. Finally, complete plantlets were obtained on a hormone-free MS solid medium.
从绞股蓝的悬浮培养物中分离出原生质体，并采用液体和看护培养法在不同培养基中进行培养。4天内观察到细胞分裂，4周内形成了微愈伤组织。在含有1mg/L激动素（KT）和0.5mg/L吲哚乙酸（IAA）的MS固体培养基上，原生质体来源的愈伤组织分化成胚。在含有1mg/L 6-苄基腺嘌呤（6-BA）和0.5mg/L吲哚乙酸（IAA）的MS培养基上形成了茎和叶。最后，在无激素的MS固体培养基上获得了完整的植株。
