Formaggio F, Crisma M, Rossi P, Scrimin P, Kaptein B, Broxterman Q B, Kamphuis J, Toniolo C
Department of Organic Chemistry, University of Padova, CNR Centre CSB, Italy.
Chemistry. 2000 Dec 15;6(24):4498-504. doi: 10.1002/1521-3765(20001215)6:24<4498::aid-chem4498>3.0.co;2-4.
Two water-soluble 3(10)-helical peptides are synthesized and fully characterized for the first time. The sequence of these terminally blocked heptamers comprises two residues of the Calpha-trisubstituted alpha-amino acid 2-amino-3-[1-(1,4,7-triazacyclononyl)]propanoic acid and five residues of a Calpha-tetrasubstituted alpha-amino acid (either alpha-aminoisobutyric acid or isovaline). Using CD and NMR techniques we were able to show that both heptapeptides are well structured in water, and that the type of conformation adopted is indeed the ternary 3(10)-helix.
首次合成并全面表征了两种水溶性3(10)-螺旋肽。这些末端封闭的七聚体的序列包含两个α-三取代α-氨基酸2-氨基-3-[1-(1,4,7-三氮杂环壬基)]丙酸的残基和五个α-四取代α-氨基酸（α-氨基异丁酸或异缬氨酸）的残基。使用圆二色光谱（CD）和核磁共振（NMR）技术，我们能够证明这两种七肽在水中结构良好，并且所采用的构象类型确实是三元3(10)-螺旋。
