De Herdt E, Slegers H, Kondo M
Eur J Biochem. 1979 Jun 1;96(3):423-30. doi: 10.1111/j.1432-1033.1979.tb13054.x.
The cytoplasm of the cryptobiotic Artemia salina gastrula contains a large quantity of a unique 19-S complex. This particle is a specific aggregated form of a 27 000-Mr protein, having a molecular weight of 10(6) and an apparent buoyant density of 1.25 -- 1.26 g/cm3 in sucrose and 1.31 g/cm3 in CsCl. The relative quantity of this 19-S complex decreases significantly with respect to 80-S monoribosomes during the postgastrula development. Biochemical and immunological studies indicate that the 27 000-Mr protein is one of the RNA-binding proteins [Ovchinnikov et al., FEBS Lett. 88, 21 -- 26 (1978)] but is absent in the protein components associated with poly(A)-containing ribonucleoprotein complexes. The possibility is also suggested that the 27 000-Mr protein and Artemia elongation factor eEF-Ts might be related to each other on the basis of amino acid composition and immunological cross-reactivity.
处于隐生状态的卤虫原肠胚的细胞质中含有大量独特的19-S复合物。该颗粒是一种分子量为27000的蛋白质的特定聚集形式，分子量为10(6)，在蔗糖中的表观浮力密度为1.25 -- 1.26 g/cm3，在氯化铯中的表观浮力密度为1.31 g/cm3。在原肠胚后期发育过程中，相对于80-S单核糖体，这种19-S复合物的相对量显著减少。生化和免疫学研究表明，分子量为27000的蛋白质是RNA结合蛋白之一[奥夫钦尼科夫等人，《欧洲生物化学学会联合会快报》88，21 -- 26(1978)]，但在与含聚腺苷酸的核糖核蛋白复合物相关的蛋白质成分中不存在。基于氨基酸组成和免疫交叉反应性，还提出了分子量为27000的蛋白质与卤虫延伸因子eEF-Ts可能相互关联的可能性。
