卤虫隐生胚胎的信使核糖核蛋白复合体
Messenger ribonucleoprotein complexes of cryptobiotic embryos of Artemia salina.
作者信息
Slegers H, Kondo M
出版信息
Nucleic Acids Res. 1977 Mar;4(3):625-39. doi: 10.1093/nar/4.3.625.
Poly(A)-containing ribonucleoprotein (poly(A)+-RNP) particles in the post-mitochondrial supernatant of cryptobiotic embryos of Artemia salina were characterized by hybridization to [3H]-poly(U). By sucrose isopycnic centrifugation, approximately 2/3 of poly(A)+-RNPs was found to band at 1.27-1.30 (g/cm3) and the rest 1+/3 at 1.20-1.23 (g/cm3) and below 1.20 (g/cm3). The 1.27-1.30 RNPs could be separated into two density classes, 1.27-1.28 and 1.30 (g/cm3) respectively. The latter RNP class was apparently complexed with ribosomal components because they were completely converted to the former RNP class (free RNPs) by 25 mM EDTA treatment. Further, the 1.30 (g/cm3) RNPs were resolved into several RNP species having sedimentation coefficients above 50 S. which were transformed mostly to 20-30 S rnps in the presence of 25 mM EDTA. The free 20-30 S RNPs contained 8-14 S poly(A)+-RNAs, having the highest template activity in a wheat embryo cell-free system, whereas the 1.20-1.23 poly(A)+-RNPs consisted of 10 S and 16 S RNPs, both of which contained 4 S poly(A)-containing sequences without any template activity.
通过与[3H]-聚尿嘧啶（poly(U)）杂交，对卤虫隐生胚胎线粒体后上清液中含聚腺苷酸（Poly(A)）的核糖核蛋白（poly(A)+-RNP）颗粒进行了表征。通过蔗糖等密度离心发现，约2/3的poly(A)+-RNPs在1.27 - 1.30（g/cm³）处形成条带，其余1/3在1.20 - 1.23（g/cm³）以及低于1.20（g/cm³）处形成条带。1.27 - 1.30的RNPs可分为两个密度类别，分别为1.27 - 1.28和1.30（g/cm³）。后一类RNP显然与核糖体成分复合，因为经25 mM EDTA处理后它们完全转化为前一类RNP（游离RNPs）。此外，1.30（g/cm³）的RNPs可解析为几种沉降系数高于50 S的RNP种类，在25 mM EDTA存在的情况下，它们大多转化为20 - 30 S的RNPs。游离的20 - 30 S RNPs含有8 - 14 S的poly(A)+-RNAs，在小麦胚无细胞体系中具有最高的模板活性，而1.20 - 1.23的poly(A)+-RNPs由10 S和16 S的RNPs组成，二者均含有4 S的含poly(A)序列，且均无任何模板活性。
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