钴（III）多吡啶配合物的DNA结合与光裂解研究：

DNA-binding and photocleavage studies of cobalt(III) polypyridyl complexes:

作者信息

Zhang Q L, Liu J G, Chao H, Xue G Q, Ji L N

机构信息

Department of Chemistry, Zhongshan University, Guangzhou, PR China.

出版信息

J Inorg Biochem. 2001 Jan 1;83(1):49-55. doi: 10.1016/s0162-0134(00)00132-x.

DOI:10.1016/s0162-0134(00)00132-x

PMID:11192699

Abstract

Two complexes of [Co(phen)2IP]3+ (IP=imidazo[4,5-f][l,10]phenanthroline) and [Co(phen)2PIP]3+ (PIP=2-phenylimidazo[4,5-f][1,10]phenanthroline) have been synthesized and characterized by UV/VIS, IR, EA and mass spectra. The binding of the two complexes with calf thymus DNA has been investigated by absorption spectroscopy, cyclic voltammetry, viscosity measurements and DNA cleavage assay. The spectroscopic studies together with cyclic voltammetry and viscosity experiments support that both of the complexes bind to CT DNA by intercalation via IP or PIP into the base pairs of DNA. [Co(phen)2PIP]3+ binds more avidly to CT DNA than [Co(phen)2IP]3+, which is consistent with the extended planar and pi system of PIP. Noticeably, the two complexes have been found to be efficient photosensitisers for strand scissions in plasmid DNA.

摘要

已合成了[Co(phen)2IP]3+（IP = 咪唑并[4,5-f][1,10]菲咯啉）和[Co(phen)2PIP]3+（PIP = 2-苯基咪唑并[4,5-f][1,10]菲咯啉）的两种配合物，并通过紫外可见光谱、红外光谱、元素分析和质谱对其进行了表征。通过吸收光谱、循环伏安法、粘度测量和DNA切割试验研究了这两种配合物与小牛胸腺DNA的结合情况。光谱研究以及循环伏安法和粘度实验表明，两种配合物均通过IP或PIP插入DNA碱基对以嵌入方式与CT DNA结合。[Co(phen)2PIP]3+比[Co(phen)2IP]3+更紧密地结合到CT DNA上，这与PIP的扩展平面和π体系一致。值得注意的是，已发现这两种配合物是质粒DNA链断裂的有效光敏剂。