Jin L, Yang P
State Key Laboratory of Coordination Chemistry, Nanjing University, People's Republic of China.
J Inorg Biochem. 1997 Nov 1;68(2):79-83. doi: 10.1016/s0162-0134(97)00004-4.
The complex of Co(phen)2dppz3+ (where phen is ophenanthroline, and dppz is dipyrido [3, 2-a: 2', 3'-c] phenazine) has been synthesized. This complex was characterized by elemental analysis, molar conductivity, IR and 1H NMR spectroscopy. The interaction of the complex with calf thymus DNA has been studied using absorption and emission spectroscopy, DNA melting techniques, cyclic voltammetric, viscosity, and electrophoresis measurements. The compound shows absorption hypochromicity, fluorescence enhancement, DNA melting temperature, and the specific viscosity increased when binding to calf thymus DNA. CV measurement shows that the shifts in oxidation-reduction potential and change in peak current with addition of DNA. The complex is also shown to be more efficient photosensitisers for single strand breaks in plasmid DNA.
已合成了Co(phen)2dppz3+配合物（其中phen为邻菲罗啉，dppz为二吡啶并[3,2 - a:2',3'- c]吩嗪）。该配合物通过元素分析、摩尔电导率、红外光谱和1H核磁共振光谱进行了表征。利用吸收光谱和发射光谱、DNA熔解技术、循环伏安法、粘度和电泳测量研究了该配合物与小牛胸腺DNA的相互作用。该化合物与小牛胸腺DNA结合时表现出吸收减色性、荧光增强、DNA熔解温度升高以及比粘度增加。循环伏安测量表明，加入DNA后氧化还原电位发生偏移且峰值电流发生变化。该配合物还被证明是用于质粒DNA单链断裂的更有效的光敏剂。
