Chen Hui-li, Yang Pin
Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2002 Dec;22(6):983-6.
Two complexes ofCo(phen)2PIP3.H2O and Co(phen)2HPIP3.2H2O have been synthesized(PIP = 2-imidazo[4,5-f][1,10]phenanthroline, HPIP = 2-(2-hydroxyphenyl)-imidazo [4,5-f][1,10]phenanthroline) and characterized by elemental analysis, UV, IR and 1H NMR spectroscopy. The interaction of the complexes with calf thymus DNA have been studied using absorption and emission spectroscopy, DNA melting techniques and cyclic voltammetric. The compounds show absorption hypochromicity, fluorescence enhancement, DNA melting increasement when binding to CT.DNA. CV measurement shows that the shift in reduction potential and the change in peak current with addition of DNA. These results prove the compounds insert DNA base pairs. The shift of peak potential indicates the ion interaction mode between the drug and DNA. The binding constants of the compounds to DNA are 1.8 x 10(4) and 4.37 x 10(4) respectively.
合成了Co(phen)2PIP3·H2O和Co(phen)2HPIP3·2H2O两种配合物（PIP = 2-咪唑并[4,5-f][1,10]菲咯啉，HPIP = 2-(2-羟基苯基)-咪唑并[4,5-f][1,10]菲咯啉），并通过元素分析、紫外光谱、红外光谱和1H NMR光谱对其进行了表征。利用吸收光谱和发射光谱、DNA熔解技术和循环伏安法研究了配合物与小牛胸腺DNA的相互作用。这些化合物与CT-DNA结合时表现出吸收减色性、荧光增强和DNA熔解增加。循环伏安测量表明，加入DNA后还原电位发生偏移，峰电流发生变化。这些结果证明化合物插入了DNA碱基对。峰电位的偏移表明药物与DNA之间的离子相互作用模式。化合物与DNA的结合常数分别为1.8×10(4)和4.37×10(4)。
