意大利牛泰勒虫和巴贝斯虫种类的综合分子诊断

Integrated molecular diagnosis of Theileria and Babesia species of cattle in Italy.

作者信息

Sparagano O, Loria G R, Gubbels M J, De Vos A P, Caracappa S, Jongejan F

机构信息

Department of Biological Sciences, Heriot-Watt University, Riccarton Park, Edinburgh EH14 4AS, Scotland.

出版信息

Ann N Y Acad Sci. 2000;916:533-9. doi: 10.1111/j.1749-6632.2000.tb05332.x.

DOI:10.1111/j.1749-6632.2000.tb05332.x

PMID:11193668

Abstract

A reverse line blot hybridization (RLB) test was developed to specifically identify six Theileria spp. (T. annulata, T. parva, T. mutans, T. velifera, T. taurotragi, and T. buffeli/orientalis) and three Babesia spp. (B. bovis, B. bigemina, and B. divergens). No cross reaction was observed with other livestock pathogens (such as Anaplasma marginale, A. centrale, A. ovis, Cowdria ruminantium, Trypanosoma brucei, T. congolense, and T. vivax). This method was used to test bovine blood samples collected in Sicily in April and November, 1998. Preliminary results indicated that T. annulata and T. buffeli/orientalis were the main species observed in cattle blood. Babesia species represented 1.8% and 23.5% in April and November, respectively.

摘要

开发了一种反向线印迹杂交（RLB）试验，以特异性鉴定六种泰勒虫属物种（环形泰勒虫、小泰勒虫、突变泰勒虫、韦氏泰勒虫、南非水牛泰勒虫和水牛泰勒虫/东方泰勒虫）和三种巴贝斯虫属物种（牛巴贝斯虫、双芽巴贝斯虫和分歧巴贝斯虫）。未观察到与其他家畜病原体（如边缘无浆体、中央无浆体、绵羊无浆体、反刍动物考德里氏体、布氏锥虫、刚果锥虫和活泼锥虫）的交叉反应。该方法用于检测1998年4月和11月在西西里岛采集的牛血样本。初步结果表明，环形泰勒虫和水牛泰勒虫/东方泰勒虫是在牛血中观察到的主要物种。巴贝斯虫属物种在4月和11月分别占1.8%和23.5%。