[使用反向线印迹法对埃尔津詹地区牛体内环形泰勒虫和水牛泰勒虫/东方泰勒虫的调查]

[Survey of T. annulata and T. buffeli/orientalis in cattle in the region of Erzincan using reverse line blotting].

作者信息

Altay Kürşat, Aktaş Münir, Dumanli Nazir

机构信息

Firat Universitesi, Veteriner Fakültesi Parazitoloji Anabilim Dali, Elaziğ, Turkey.

出版信息

Turkiye Parazitol Derg. 2007;31(2):94-7.

PMID:17594645

Abstract

This study was carried out to investigate Theileria annulata and T. buffeli/orientalis in cattle in the region of Erzincan using reverse line blotting (RLB) and microscopical examination. A total of 123 blood samples and thin blood smears were collected from cattle in distinct locations. Thin blood smears were microscopically examined for Theileria piroplasms. The 18S SSU rRNA gene in the DNA of Theileria spp extracted from blood was amplified and used in RLB. For this purpose, PCR products were hybridized with specific probes for over-all Theileria spp., T. annulata and T. buffeli/orientalis as well as Babesia spp. While Theileria spp. were observed in 14 out of 123 cattle, (11.38 %) during microscopical examination, T. annulata was detected in 19 (15.45%) cattle and T. buffeli/orientalis, in 12 (9.76%) by RLB, respectively. Mixed infection was also detected in three samples.

摘要

本研究旨在利用反向线印迹法（RLB）和显微镜检查，调查埃尔津詹地区牛体内的环形泰勒虫以及水牛泰勒虫/东方泰勒虫。从不同地点的牛身上共采集了123份血液样本和薄血涂片。对薄血涂片进行显微镜检查，以查找泰勒虫梨形虫体。从血液中提取的泰勒虫属DNA中的18S小亚基核糖体RNA（rRNA）基因经扩增后用于反向线印迹法。为此，将聚合酶链反应（PCR）产物与针对所有泰勒虫属、环形泰勒虫、水牛泰勒虫/东方泰勒虫以及巴贝斯虫属的特异性探针进行杂交。在显微镜检查中，123头牛中有14头（11.38%）检测到泰勒虫属，通过反向线印迹法分别在19头（15.45%）牛中检测到环形泰勒虫，在12头（9.76%）牛中检测到水牛泰勒虫/东方泰勒虫。在三个样本中还检测到混合感染。