采用柱端安培检测的毛细管区带电泳法监测人血清转铁蛋白。
Monitoring human serum transferrin by capillary zone electrophoresis with end-column amperometric detection.
作者信息
Dong Q, Yu D, Ye X, Jin W
机构信息
Laboratory of Analytical Science, School of Chemistry, Shandong University, Jinan, China.
出版信息
Electrophoresis. 2001 Jan;22(1):128-33. doi: 10.1002/1522-2683(200101)22:1<128::AID-ELPS128>3.0.CO;2-L.
Capillary zone electrophoresis was employed for the determination of human serum transferrin using end-column amperometric detection with a carbon fiber microelectrode at a constant potential of 1.9 V vs. saturated calomel electrode (SCE). The optimum conditions of separation and detection are 7.5 x 10(-4) mol/L Tris-3.44 x 10(-4) mol/L HCl for the buffer solution, 20 kV for the separation voltage, 5 kV and 10 s for the injection voltage and the injection time, respectively. The limit of detection is 6.7 x 10(-8) mol/L or 440 amol (S/N = 2). The relative standard deviations are 0.67% for the migration time and 1.5% for the electrophoretic peak current. The method was applied to the determination of transferrin in human serum. The recovery is between 93-104%.
采用毛细管区带电泳法，以碳纤维微电极作为柱端安培检测器，在相对于饱和甘汞电极（SCE）为1.9 V的恒定电位下测定人血清转铁蛋白。分离和检测的最佳条件为：缓冲溶液为7.5×10⁻⁴ mol/L Tris - 3.44×10⁻⁴ mol/L HCl，分离电压为20 kV，进样电压和进样时间分别为5 kV和10 s。检测限为6.7×10⁻⁸ mol/L或440 amol（信噪比S/N = 2）。迁移时间的相对标准偏差为0.67%，电泳峰电流的相对标准偏差为1.5%。该方法用于测定人血清中的转铁蛋白，回收率在93%至104%之间。
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