一种采用柱端安培检测的毛细管区带电泳法定量测定吡哌酸的新方法。
A new method of quantification of pipemidic-acid by capillary zone electrophoresis with end-column amperometric detection.
作者信息
Jin W, Yu D, Dong Q, Ye X
机构信息
Laboratory of Analytical Science, School of Chemistry, Shandong University, Jinan, China.
出版信息
Electrophoresis. 2000 Mar;21(5):925-9. doi: 10.1002/(SICI)1522-2683(20000301)21:5<925::AID-ELPS925>3.0.CO;2-T.
Capillary zone electrophoresis was employed for the determination of pipemidic acid using an end-column amperometric detection with a carbon fiber microdisk array electrode, at a constant potential of -1.10 V vs. saturated calomel electrode. The optimum conditions of separation and detection were 1.2 x 10(-4) mol/LNaOAc - 8.8 x 10(-4) mol/ LHOAc for the buffer solution, 20 kV for the separation voltage, 5 kV and 10 s for the injection voltage and the injection time. The limit of detection was 1.05 x 10(-7) mol/L or 189 amol (S/N=3). The relative standard deviation was 0.31% for the migration time and 2.0% for the electrophoretic peak current. The method was applied to determining pipemidic acid in human serum.
采用毛细管区带电泳法，以碳纤维微盘阵列电极作为柱端安培检测器，在相对于饱和甘汞电极 -1.10 V 的恒定电位下测定吡哌酸。分离和检测的最佳条件为：缓冲溶液为 1.2×10⁻⁴ mol/L 醋酸钠 - 8.8×10⁻⁴ mol/L 醋酸，分离电压为 20 kV，进样电压为 5 kV，进样时间为 10 s。检测限为 1.05×10⁻⁷ mol/L 或 189 amol（信噪比 S/N = 3）。迁移时间的相对标准偏差为 0.31%，电泳峰电流的相对标准偏差为 2.0%。该方法应用于测定人血清中的吡哌酸。
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