Giedrojć J, Klimiuk M, Janczarek B, Zabicki D, Kłoczko J
Klinika Hematologii Akademii Medycznej w Białymstoku.
Przegl Lek. 2000;57(9):459-64.
The aim of our study was the assessment whether internal peroxidase of arachidonic acid and/or prostaglandin can be transferred from platelets to monocytes. Monocytes were isolated from whole blood according to the method of Böyum. The monocytes cultures were led in the fluid environment, on the base of RPMI 1640 supplemented with autologous serum. Platelets were isolated from whole blood according to the method of Pinckard. During interaction of monocytes and platelets in the presence of indometacin we ascertained the increased of TXB2 concentrations in supper-natant. Incubation of monocytes in cultures stimulated by LPS in presence of indomethacin with platelets did not cause increased secretion of TXB2. Incubation of platelets with indomethacin in 36 h culture of monocytes stimulated by LPS caused increase concentration of 6-keto-PGF1alfa. The results of our study may suggest the presence of the one way transformation of the arachidonic acid from platelets to monocytes.
我们研究的目的是评估花生四烯酸和/或前列腺素的内源性过氧化物酶是否可以从血小板转移至单核细胞。单核细胞根据Böyum的方法从全血中分离得到。单核细胞培养在以添加自体血清的RPMI 1640为基础的液体环境中进行。血小板根据Pinckard的方法从全血中分离得到。在吲哚美辛存在的情况下单核细胞与血小板相互作用期间，我们确定上清液中TXB2浓度增加。在吲哚美辛存在的情况下，脂多糖刺激的单核细胞培养物中单核细胞与血小板共孵育并未导致TXB2分泌增加。在脂多糖刺激的单核细胞36小时培养过程中，血小板与吲哚美辛共孵育导致6-酮-PGF1α浓度增加。我们的研究结果可能提示花生四烯酸存在从血小板到单核细胞的单向转化。
