Giedrojć J, Czaczkowska T, Bielawiec M
Department of Haematology, Medical School of Białystok, Poland.
Folia Haematol Int Mag Klin Morphol Blutforsch. 1990;117(3):479-84.
The importance of circulating blood cells has been further emphasized as monocytes may also contribute to the pathogenesis of atherosclerosis. The pathological changes in atheroma may be partly mediated by metabolites of arachidonic acid. The aim of this study was determining thromboxane B2 concentrations in the supernatants of monocytes cultures and its influence on platelet aggregation. Human blood monocytes were recovered according to the procedure of Böyum and incubated with LPS. It was shown that monocytes produced thromboxane A2. The culture supernatant of monocytes from 36 h cultivation exerts an independent effect on platelet aggregation, which has been modified by inhibitors of aggregating factors. The results may indicate the influence of monocytes on AA metabolism in particular TXB2 production, platelet function and indirectly on the atherosclerotic process.
循环血细胞的重要性已得到进一步强调，因为单核细胞也可能参与动脉粥样硬化的发病机制。动脉粥样瘤的病理变化可能部分由花生四烯酸的代谢产物介导。本研究的目的是测定单核细胞培养上清液中血栓素B2的浓度及其对血小板聚集的影响。根据Böyum的方法分离人血单核细胞，并与脂多糖一起孵育。结果表明单核细胞可产生血栓素A2。培养36小时的单核细胞培养上清液对血小板聚集有独立作用，该作用已被聚集因子抑制剂所改变。这些结果可能表明单核细胞对花生四烯酸代谢特别是血栓素B2产生、血小板功能以及动脉粥样硬化过程有间接影响。
