Leclercq P D, McKenzie J E, Graham D I, Gentleman S M
Department of Neuroinflammation, Division of Neuroscience and Psychological Medicine, Imperial College School of Medicine, London, UK.
J Neurotrauma. 2001 Jan;18(1):1-9. doi: 10.1089/089771501750055721.
Amyloid precursor protein (APP) accumulation is a sensitive marker for the axonal damage that is commonly seen in the brain as the result of head injury. This form of damage is particularly associated with midline structures such as the corpus callosum, although it is not clear whether some areas are more susceptible than others. The aim of this study was to determine if there was a differential distribution of axonal injury throughout the corpus callosum after head injury in an unselected group of cases. Coronal tissue sections from eight cases were taken at different levels through the corpus callosum, including the genu, body, and splenium. The sections were immunostained with an antibody to APP, and the amount of axonal damage at the different levels was quantified using computer image analysis to build up a rostro-caudal profile for each case. The profiles revealed a significantly higher APP load in caudal parts of the corpus callosum. This supports previous nonquantitative reports in the literature and has important implications in terms of choosing where tissue should be sampled to maximize the chance of detecting axonal injury post mortem.
淀粉样前体蛋白（APP）的积累是轴突损伤的一个敏感标志物，这种损伤在因头部受伤导致的大脑中很常见。这种损伤形式尤其与胼胝体等中线结构有关，尽管尚不清楚某些区域是否比其他区域更易受损。本研究的目的是确定在一组未经挑选的病例中，头部受伤后胼胝体中轴突损伤的分布是否存在差异。从八例病例中获取了通过胼胝体不同水平的冠状组织切片，包括膝部、体部和压部。切片用抗APP抗体进行免疫染色，并使用计算机图像分析对不同水平的轴突损伤量进行量化，以建立每个病例的前后位分布图。这些分布图显示胼胝体尾部的APP负荷明显更高。这支持了文献中先前的非定量报告，并且在选择死后组织采样部位以最大化检测轴突损伤的机会方面具有重要意义。
