一种新型潜在反义分子2-5A-(5')-DNA-(3')-PNA嵌合体的合成及其与RNA酶L的结合和激活

Synthesis and RNAse L binding and activation of a 2-5A-(5')-DNA-(3')-PNA chimera, a novel potential antisense molecule.

作者信息

Verheijen J C, Chen L, Bayly S F, Torrence P F, van der Marel G A, van Boom J H

机构信息

Leiden Institute of Chemistry, Gorlaeus Laboratories, The Netherlands.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2000 Oct-Dec;19(10-12):1821-30. doi: 10.1080/15257770008045463.

DOI:10.1080/15257770008045463

PMID:11200276

Abstract

Fully automated solid-phase synthesis gave access to a hybrid in which 5'-phosphorylated-2'-5'-linked oligoadenylate (2-5A) is connected to the 5'-terminus of DNA which, in turn, is linked at the 3'-end to PNA [2-5A-(5')-DNA-(3')-PNA chimera]. This novel antisense molecule retains full RNase L activation potency while suffering only a slight reduction in binding affinity.

摘要

全自动化固相合成得到了一种杂合体，其中5'-磷酸化的2'-5'-连接的寡聚腺苷酸（2-5A）连接到DNA的5'-末端，而DNA的3'-末端又与肽核酸相连[2-5A-(5')-DNA-(3')-肽核酸嵌合体]。这种新型反义分子保留了完整的核糖核酸酶L激活能力，而结合亲和力仅略有降低。