双头2-5A反义嵌合体的合成及其激活人核糖核酸酶L的能力。

Synthesis of double-headed 2-5A-antisense chimeras and their ability to activate human RNase L.

作者信息

Ueno Yoshihito, Okatani Shusaku, Yamada Yuuki, Kitade Yukio

机构信息

Department of Biomolecular Science, Faculty of Engineering, Gifu University, Yanagido, Gifu 501-1193, Japan.

出版信息

Nucleic Acids Res Suppl. 2003(3):63-4. doi: 10.1093/nass/3.1.63.

DOI:10.1093/nass/3.1.63

PMID:14510381

Abstract

The synthesis of a novel 2-5A-antisense chimera having two molecules of 2-5A tetramer at the 5'-terminus of the antisense moiety with an ethylene glycol linker is described. The ability of the synthesized 2-5A antisense chimeras to activate RNase L was estimated by monitoring the cleavage of a target RNA by the activated RNase L. It was found that the double-headed 2-5A-antisense chimera linked with two molecules of a butanediol linker more efficiently cleaved the target RNA as compared with the single-headed 2-5A-antisense chimera and the double-headed 2-5A-antisense chimera linked with a molecule of the butanediol linker.

摘要

描述了一种新型的2-5A反义嵌合体的合成，该嵌合体在反义部分的5'-末端具有两个2-5A四聚体分子，并通过乙二醇连接子连接。通过监测活化的RNase L对靶RNA的切割来评估合成的2-5A反义嵌合体激活RNase L的能力。结果发现，与单头2-5A反义嵌合体和与一个丁二醇连接子连接的双头2-5A反义嵌合体相比，与两个丁二醇连接子分子连接的双头2-5A反义嵌合体更有效地切割靶RNA。