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A new strategy of discrimination of a point mutation by tandem of short oligonucleotides.

作者信息

Pyshnyi D V, Lokhov S G, Podyminogin M A, Ivanova E M, Zarytova V F

机构信息

Novosibirsk Institute of Bioorganic Chemistry, Russia.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2000 Oct-Dec;19(10-12):1931-41. doi: 10.1080/15257770008045469.

DOI:10.1080/15257770008045469
PMID:11200282
Abstract

A new strategy based on the use of cooperative tandems of short oligonucleotide derivatives (TSOD) has been proposed to discriminate a "right" DNA target from a target containing a single nucleotide discrepancy. Modification of a DNA target by oligodeoxyribonucleotide reagents was used to characterize their interaction in the perfect and mismatched complexes. It is possible to detect any nucleotide changes in the binding sites of the target with the short oligonucleotide reagent. In the presence of flanking di-3',5'-N-(2-hydroxyethyl)phenazinium derivatives of short oligonucleotides (effectors) the tetranucleotide alkylating reagent modifies DNA target efficiently and site-specifically only in the perfect complex and practically does not modify it in the mismatched complex. It has been shown that TSOD is much more sensitive tool for the detection of a point mutation in DNA as compared to a longer oligonucleotides.

摘要

相似文献

1
A new strategy of discrimination of a point mutation by tandem of short oligonucleotides.
Nucleosides Nucleotides Nucleic Acids. 2000 Oct-Dec;19(10-12):1931-41. doi: 10.1080/15257770008045469.
2
[Nucleic acids interactions with short oligonucleotide derivatives. II. Tandem of short oligonucleotides as highly sensitive system for identification of single base substitutions in target DNA].
Bioorg Khim. 1997 Jul;23(7):561-8.
3
[Interaction of short nucleotide derivatives with nucleic acids. IV. Modification of DNA by an alkylating tetranucleotide reagents in the presence of effectors in perfect and imperfect complexes].
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[Interaction of short oligonucleotides derivatives with nucleic acids. VI. Discrimination of mismatch-containing complexes upon ligation of a short oligonucleotide tandem on DNA template].[短寡核苷酸衍生物与核酸的相互作用。VI. 基于DNA模板上短寡核苷酸串联连接对含错配复合物的区分]
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[Site-directed chemical restriction of a single-stranded DNA fragment by an alkylating derivative of the tetranucleotide d(pApGpCpA) in the presence of tetranucleotide effectors].
Bioorg Khim. 1992 Jul;18(7):895-900.
8
[Effective selective modification of a single-stranded fragment of DNA with alkylating derivatives of short oligodeoxyribonucleotides in the presence of reaction effectors N-(2-hydroxyethyl)phenazine derivatives of oligodeoxyribonucleotides].[在反应效应物寡脱氧核糖核苷酸的N-(2-羟乙基)吩嗪衍生物存在下，用短寡脱氧核糖核苷酸的烷基化衍生物对DNA单链片段进行有效的选择性修饰]
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[Interaction of derivatives of short oligonucleotides with nucleic acids. I. Effect of various types of effectors on alkylation of DNA-targets].
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Cooperative interactions of the oligodeoxyribonucleotides on the complementary template. The influence of chemical groups and mismatched nucleotides at the 5'- and 3'-ends of oligonucleotides on the parameters of cooperativity.寡脱氧核糖核苷酸在互补模板上的协同相互作用。寡核苷酸5'端和3'端的化学基团及错配核苷酸对协同性参数的影响。
J Biomol Struct Dyn. 1997 Oct;15(2):369-80. doi: 10.1080/07391102.1997.10508199.

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