Pyshnyĭ D V, Lokhov S G, Ivanova E M, Zarytova V F
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, Russia.
Bioorg Khim. 1998 Mar;24(3):201-10.
The influence of effectors [octanucleotides and their 3',5'-di-N-(2-hydroxyethyl)phenazinium derivatives] on the modification of a target DNA by alkylating oligonucleotide derivatives forming duplexes of different stability with the target ws studied. It is shown that, being in tandem complexes immediately adjacent to the reactive group of an oligonucleotide reagent possessing a high hybridization capacity, the effector, on the one hand, enhances the stability of the reagent target duplex, and on the other hand, changes the site-specificity of alkylation and decreases the efficiency of the target modification at temperatures that provide a high extent of the target association with the reagent. Conversely, in the case of oligonucleotide reagents forming weak complexes with the target, effectors enhance both the stability of the target.reagent duplex and the extent of the target throughout the temperature range tested. The data indicate that the varying influence of effectors on the target modification by reagents with different hybridization capacities is due to conformational features of the target reagent duplexed regions. Increasing the rigidity of the target.reagent duplex reduces the efficiency of the target modification in tandem complexes.
研究了效应物[八聚核苷酸及其3'，5'-二-N-（2-羟乙基）吩嗪鎓衍生物]对通过与靶标形成不同稳定性双链体的烷基化寡核苷酸衍生物修饰靶标DNA的影响。结果表明，效应物一方面处于紧邻具有高杂交能力的寡核苷酸试剂反应基团的串联复合物中，增强了试剂-靶标双链体的稳定性，另一方面改变了烷基化的位点特异性，并在使靶标与试剂高度缔合的温度下降低了靶标修饰的效率。相反，对于与靶标形成弱复合物的寡核苷酸试剂，效应物在整个测试温度范围内增强了靶标-试剂双链体的稳定性以及靶标的缔合程度。数据表明，效应物对具有不同杂交能力的试剂修饰靶标的不同影响是由于靶标-试剂双链区域的构象特征所致。增加靶标-试剂双链体的刚性会降低串联复合物中靶标修饰的效率。
