Rosenberger J K, Klopp S, Krauss W C
Avian Dis. 1975 Jan-Mar;19(1):142-9.
Four isolants of Newcastle disease virus (NDV) obtained from free-flying Canada geese in the Atlantic flyway were characterized and compared with the B1, LaSota, Roakin, and Texas-GB strains of NDV. The 4 isolants were identified as lentogenic strains on the basis of embryo mean death times of greater than 100 hours and relative lack of pathogenicity for 1-day-old and 3-week-old chickens. The hemagglutinin of 2 of the isolants was stable at 56 C for 15 minutes, 1 for 30 minutes, and the other for at least 2 hours. When held at 4 C for 24 hours, none of the 4 isolants could be eluted from chicken erythrocytes. All 4 of the isolants produced plaques in chicken embryo fibroblast cell cultures without additives, whereas the B1 and LaSota strains did not. The 4 lentogenic NDV isolants from Canada geese differed in many respects from currently used commerical vaccine strains (B1 and LaSota).
对从大西洋飞行路线上自由飞行的加拿大鹅中分离得到的4株新城疫病毒（NDV）进行了特性鉴定，并与NDV的B1、LaSota、Roakin和Texas-GB毒株进行了比较。根据胚胎平均死亡时间大于100小时以及对1日龄和3周龄鸡相对缺乏致病性，将这4株分离株鉴定为弱毒株。其中2株分离株的血凝素在56℃下15分钟稳定，1株30分钟稳定，另一株至少2小时稳定。在4℃下保存24小时后，这4株分离株均不能从鸡红细胞上洗脱下来。所有4株分离株在无添加剂的鸡胚成纤维细胞培养物中均可产生蚀斑，而B1和LaSota毒株则不能。这4株来自加拿大鹅的弱毒NDV分离株在许多方面与目前使用的商业疫苗毒株（B1和LaSota）不同。
