鸡毒支原体细胞黏附素MGC2的特性分析，该黏附素与肺炎支原体30千道尔顿蛋白P30及生殖支原体P32具有同源性。
Characterization of MGC2, a Mycoplasma gallisepticum cytadhesin with homology to the Mycoplasma pneumoniae 30-kilodalton protein P30 and Mycoplasma genitalium P32.
作者信息
Hnatow L L, Keeler C L, Tessmer L L, Czymmek K, Dohms J E
机构信息
Department of Animal and Food Sciences, College of Agriculture and Natural Resources, University of Delaware, Newark, Delaware 19717-1303, USA.
出版信息
Infect Immun. 1998 Jul;66(7):3436-42. doi: 10.1128/IAI.66.7.3436-3442.1998.
Abstract
A second cytadhesin-like protein, MGC2, was identified in the avian respiratory pathogen Mycoplasma gallisepticum. The 912-nucleotide mgc2 gene encodes a 32.6-kDa protein with 40.9 and 31.4% identity with the M. pneumoniae P30 and M. genitalium P32 cytadhesins, respectively. Functional studies with reverse transcription-PCR, immunoblotting, double-sided immunogold labeling, and attachment inhibition assays demonstrated homology to the human mycoplasmal P30 and P32 cytadhesins. These findings suggest that there is a family of cytadhesin genes conserved among pathogenic mycoplasmas infecting widely divergent hosts.
摘要
在禽呼吸道病原体鸡毒支原体中鉴定出第二种细胞粘附素样蛋白MGC2。912个核苷酸的mgc2基因编码一种32.6 kDa的蛋白质，与肺炎支原体P30和生殖支原体P32细胞粘附素的同源性分别为40.9%和31.4%。通过逆转录聚合酶链反应、免疫印迹、双面免疫金标记和粘附抑制试验进行的功能研究表明，该蛋白与人类支原体P30和P32细胞粘附素有同源性。这些发现表明，在感染广泛不同宿主的致病性支原体中，存在一个细胞粘附素基因家族。
相似文献
1
Characterization of MGC2, a Mycoplasma gallisepticum cytadhesin with homology to the Mycoplasma pneumoniae 30-kilodalton protein P30 and Mycoplasma genitalium P32.鸡毒支原体细胞黏附素MGC2的特性分析，该黏附素与肺炎支原体30千道尔顿蛋白P30及生殖支原体P32具有同源性。
Infect Immun. 1998 Jul;66(7):3436-42. doi: 10.1128/IAI.66.7.3436-3442.1998.
2
Molecular characterization of the Mycoplasma gallisepticum pvpA gene which encodes a putative variable cytadhesin protein.编码一种假定的可变细胞黏附蛋白的鸡毒支原体pvpA基因的分子特征分析。
Infect Immun. 2000 Jul;68(7):3956-64. doi: 10.1128/IAI.68.7.3956-3964.2000.
3
Cross-hybridization between the cytadhesin genes of Mycoplasma pneumoniae and Mycoplasma genitalium and genomic DNA of Mycoplasma gallisepticum.肺炎支原体与生殖支原体的细胞黏附素基因之间以及鸡毒支原体基因组DNA之间的交叉杂交。
Microb Pathog. 1990 May;8(5):371-5. doi: 10.1016/0882-4010(90)90096-9.
4
Identification of the putative cytadhesin gene of Mycoplasma gallisepticum and its use as a DNA probe.鸡毒支原体假定细胞粘附素基因的鉴定及其作为DNA探针的应用。
Avian Dis. 1993 Apr-Jun;37(2):380-8.
5
Cloning and characterization of a putative cytadhesin gene (mgc1) from Mycoplasma gallisepticum.鸡毒支原体假定细胞粘附素基因（mgc1）的克隆与特性分析
Infect Immun. 1996 May;64(5):1541-7. doi: 10.1128/iai.64.5.1541-1547.1996.
6
Molecular cloning and characterization of an adherence-related operon of Mycoplasma genitalium.生殖支原体粘附相关操纵子的分子克隆与特性分析
J Bacteriol. 1995 Oct;177(20):5943-51. doi: 10.1128/jb.177.20.5943-5951.1995.
7
Molecular and biochemical analysis of a 105 kDa Mycoplasma gallisepticum cytadhesin (GapA).鸡毒支原体105 kDa细胞粘附素（GapA）的分子与生化分析
Microbiology (Reading). 1998 Nov;144 ( Pt 11):2971-2978. doi: 10.1099/00221287-144-11-2971.
8
Insights into the function of Mycoplasma pneumoniae protein P30 from orthologous gene replacement.从同源基因替换看肺炎支原体蛋白 P30 的功能。
Microbiology (Reading). 2011 Oct;157(Pt 10):2862-2870. doi: 10.1099/mic.0.052464-0. Epub 2011 Jul 21.
9
Isolation and characterization of transposon Tn4001-generated, cytadherence-deficient transformants of Mycoplasma pneumoniae and Mycoplasma genitalium.肺炎支原体和生殖支原体中转座子Tn4001产生的细胞黏附缺陷转化体的分离与鉴定
FEMS Immunol Med Microbiol. 1996 Oct;15(4):199-211. doi: 10.1111/j.1574-695X.1996.tb00086.x.
10
Use of molecular diversity of Mycoplasma gallisepticum by gene-targeted sequencing (GTS) and random amplified polymorphic DNA (RAPD) analysis for epidemiological studies.利用基因靶向测序（GTS）和随机扩增多态性DNA（RAPD）分析鸡毒支原体的分子多样性进行流行病学研究。
Microbiology (Reading). 2005 Jun;151(Pt 6):1883-1893. doi: 10.1099/mic.0.27642-0.
引用本文的文献
1
Epidemiological and molecular characterization of investigation of Mycoplasma gallisepticum of goose origin in Guangdong (China).中国广东鹅源鸡败血支原体的流行病学及分子特征研究
Poult Sci. 2025 Feb;104(2):104791. doi: 10.1016/j.psj.2025.104791. Epub 2025 Jan 8.
2
A sweeping view of avian mycoplasmas biology drawn from comparative genomic analyses.基于比较基因组分析对禽支原体生物学的全面概述。
BMC Genomics. 2025 Jan 10;26(1):24. doi: 10.1186/s12864-024-11201-5.
3
Whole genome sequencing and analysis of a goose-derived in Guangdong Province, China.中国广东省一只鹅的全基因组测序与分析。
J Vet Res. 2024 Nov 28;68(4):497-508. doi: 10.2478/jvetres-2024-0065. eCollection 2024 Dec.
4
Reviewing advancement in P30 adhesin protein provides insights for future diagnosis and treatment.回顾P30黏附蛋白的研究进展为未来的诊断和治疗提供了思路。
Front Microbiol. 2024 Dec 13;15:1515291. doi: 10.3389/fmicb.2024.1515291. eCollection 2024.
5
Designing of novel chimeric PvpA-pMGA protein of Mycoplasma gallisepticum, applicable for indirect ELISA.鸡毒支原体新型嵌合PvpA-pMGA蛋白的设计，适用于间接酶联免疫吸附测定。
J Genet Eng Biotechnol. 2022 Nov 29;20(1):160. doi: 10.1186/s43141-022-00434-0.
6
B cells oppose Mycoplasma pneumoniae vaccine enhanced disease and limit bacterial colonization of the lungs.B细胞对抗肺炎支原体疫苗增强疾病，并限制肺部细菌定植。
NPJ Vaccines. 2022 Oct 31;7(1):130. doi: 10.1038/s41541-022-00556-z.
7
Targeted sequencing analysis of Mycoplasma gallisepticum isolates in chicken layer and breeder flocks in Thailand.泰国鸡层和种鸡群中鸡毒支原体分离株的靶向测序分析。
Sci Rep. 2022 Jun 14;12(1):9900. doi: 10.1038/s41598-022-14066-4.
8
Infection strategies of mycoplasmas: Unraveling the panoply of virulence factors.支原体的感染策略：揭开毒力因子的全貌。
Virulence. 2021 Dec;12(1):788-817. doi: 10.1080/21505594.2021.1889813.
9
Current status of vaccine research, development, and challenges of vaccines for Mycoplasma gallisepticum.鸡毒支原体疫苗的研究、开发现状及面临的挑战
Poult Sci. 2020 Sep;99(9):4195-4202. doi: 10.1016/j.psj.2020.06.014. Epub 2020 Jun 27.
10
Unraveling the Global Phylodynamic and Phylogeographic Expansion of : Understanding the Origin and Expansion of This Pathogen in Ecuador.解析 的全球系统动力学和系统地理学扩张：了解该病原体在厄瓜多尔的起源与扩张。 （你提供的原文中“Unraveling the Global Phylodynamic and Phylogeographic Expansion of”后面似乎缺少具体内容）
Pathogens. 2020 Aug 19;9(9):674. doi: 10.3390/pathogens9090674.
本文引用的文献
1
Adherence of Mycoplasma gallisepticum involves variable surface membrane proteins.鸡毒支原体的黏附涉及可变表面膜蛋白。
Infect Immun. 1997 Jun;65(6):2468-71. doi: 10.1128/iai.65.6.2468-2471.1997.
2
Comparative analysis of the genomes of the bacteria Mycoplasma pneumoniae and Mycoplasma genitalium.肺炎支原体和生殖支原体细菌基因组的比较分析。
Nucleic Acids Res. 1997 Feb 15;25(4):701-12. doi: 10.1093/nar/25.4.701.
3
Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae.肺炎支原体细菌基因组的全序列分析。
Nucleic Acids Res. 1996 Nov 15;24(22):4420-49. doi: 10.1093/nar/24.22.4420.
4
Mycoplasma pneumoniae cytadherence: unravelling the tie that binds.肺炎支原体细胞黏附：解开连接的纽带
Mol Microbiol. 1996 Apr;20(2):247-53. doi: 10.1111/j.1365-2958.1996.tb02613.x.
5
Size and genomic location of the pMGA multigene family of Mycoplasma gallisepticum.鸡毒支原体pMGA多基因家族的大小及基因组定位
Microbiology (Reading). 1996 Jun;142 ( Pt 6):1429-1435. doi: 10.1099/13500872-142-6-1429.
6
Biofunctional domains of the Mycoplasma pneumoniae P30 adhesin.肺炎支原体P30黏附素的生物功能结构域。
Infect Immun. 1996 Jul;64(7):2595-601. doi: 10.1128/iai.64.7.2595-2601.1996.
7
The P200 protein of Mycoplasma pneumoniae shows common features with the cytadherence-associated proteins HMW1 and HMW3.肺炎支原体的P200蛋白与细胞黏附相关蛋白HMW1和HMW3具有共同特征。
Gene. 1996 May 24;171(1):79-82. doi: 10.1016/0378-1119(96)00014-5.
8
Sequence analysis and characterization of the hmw gene cluster of Mycoplasma pneumoniae.肺炎支原体高分子量基因簇的序列分析与特征鉴定
Gene. 1996 May 24;171(1):19-25. doi: 10.1016/0378-1119(96)00050-9.
9
Cloning and characterization of a putative cytadhesin gene (mgc1) from Mycoplasma gallisepticum.鸡毒支原体假定细胞粘附素基因（mgc1）的克隆与特性分析
Infect Immun. 1996 May;64(5):1541-7. doi: 10.1128/iai.64.5.1541-1547.1996.
10
Expression studies on four members of the pMGA multigene family in Mycoplasma gallisepticum S6.鸡毒支原体S6株中pMGA多基因家族四个成员的表达研究
Microbiology (Reading). 1995 Nov;141 ( Pt 11):3005-14. doi: 10.1099/13500872-141-11-3005.
Zotero 插件