Ergin A, Kocagöz T, Us D
Hacettepe University, Faculty of Medicine, Department of Microbiology and Clinical Microbiology, Ankara, Turkey.
Scand J Infect Dis. 2000;32(6):657-62. doi: 10.1080/003655400459586.
In this study, a total of 120 mycobacterial strains isolated from clinical specimens in Hacettepe University Hospital Clinical Pathology Laboratories were evaluated by polymerase chain reaction-restriction enzyme analysis (PRA), which analyses the common mycobacterial heat shock protein gene (hsp65). 95 of 120 strains (79.1%) were identified as Mycobacterium tuberculosis and 25 (20.8%) were identified as non-tuberculous mycobacteria (NTM). M. gordonae I and IV were the most common NTM species (3.3% each) followed by M. chelonae (2.5%). Other NTM species isolated were M. gordonae III, M. avium, M. peregrinum (1.6%), M. fortuitum, M. flavescens, M. malmoense and M. mucogenicum (0.8%). Four isolates had PRA patterns that did not match any patterns previously described. The patients who had NTM had underlying diseases; the most frequent clinical diagnosis among these was chronic obstructive pulmonary disease (COPD) and chronic renal failure. AIDS and pulmonary carcinoma were the other underlying diseases detected.
在本研究中，对从哈杰泰佩大学医院临床病理实验室的临床标本中分离出的120株分枝杆菌菌株，采用聚合酶链反应-限制性酶切分析（PRA）进行评估，该方法分析常见的分枝杆菌热休克蛋白基因（hsp65）。120株菌株中有95株（79.1%）被鉴定为结核分枝杆菌，25株（20.8%）被鉴定为非结核分枝杆菌（NTM）。戈登分枝杆菌I型和IV型是最常见的非结核分枝杆菌菌种（各占3.3%），其次是龟分枝杆菌（2.5%）。分离出的其他非结核分枝杆菌菌种有戈登分枝杆菌III型、鸟分枝杆菌、偶发分枝杆菌（1.6%）、脓肿分枝杆菌、微黄分枝杆菌、马尔默分枝杆菌和产黏液分枝杆菌（0.8%）。有4株分离株的PRA图谱与先前描述的任何图谱均不匹配。患有非结核分枝杆菌感染的患者有基础疾病；其中最常见的临床诊断是慢性阻塞性肺疾病（COPD）和慢性肾衰竭。艾滋病和肺癌是检测到的其他基础疾病。
