Doroudchi M, Kremer K, Basiri E A, Kadivar M R, Van Soolingen D, Ghaderi A A
Department of Immunology, Medical School, Shiraz University of Medical Sciences, Iran.
Scand J Infect Dis. 2000;32(6):663-8. doi: 10.1080/003655400459595.
To evaluate and compare the usefulness of IS6110-restriction fragment length polymorphism (RFLP) and spoligotyping in the epidemiology of tuberculosis in Iran, Mycobacterium tuberculosis complex strains, isolated in 2 different areas of Iran, were subjected to RFLP and spoligotyping. The average number of IS6110 copies per strain was 11 and ranged from 5 to 18 among the M. tuberculosis strains. In total, among the 62 isolates, 56 different patterns were observed. 50 strains had unique RFLP patterns (89%) and 12 (11%) revealed patterns that were found among at least 1 other isolate. Spoligotyping of 97 isolates resulted in 42 different patterns, of which 72% were found in 15 clusters. 14 (29%) out of 48 investigated isolates were resistant to 1 or more antituberculosis drugs and 57% of the resistant isolates were isolated from Afghan immigrants. Ten percent of the isolates represented the Beijing genotype, including 4 of the 14 (36%) resistant strains. Three of these resistant Beijing strains were isolated from Afghan patients. IS6110-RFLP typing could be useful for studying the epidemiology of tuberculosis in Iran. IS6110 patterns were polymorphic and the average IS6110 copy number was high.
为评估和比较IS6110限制性片段长度多态性（RFLP）和间隔寡核苷酸分型在伊朗结核病流行病学研究中的实用性，对从伊朗2个不同地区分离出的结核分枝杆菌复合群菌株进行了RFLP和间隔寡核苷酸分型。结核分枝杆菌菌株中，每株菌的IS6110拷贝数平均为11个，范围在5至18个之间。总共在62株分离菌株中观察到56种不同的模式。50株菌具有独特的RFLP模式（89%），12株（11%）显示出与至少1株其他分离菌株相同的模式。对97株分离菌株进行间隔寡核苷酸分型，得到42种不同模式，其中72%存在于15个簇中。在48株被调查的分离菌株中，14株（29%）对1种或多种抗结核药物耐药，57%的耐药菌株是从阿富汗移民中分离出来的。10%的分离菌株代表北京基因型，其中包括14株耐药菌株中的4株（36%）。这些耐药的北京菌株中有3株是从阿富汗患者中分离出来的。IS6110-RFLP分型可用于研究伊朗的结核病流行病学。IS6110模式具有多态性，且IS6110拷贝数平均值较高。
