Hartell N A, Furuya S, Jacoby S, Okada D
Pharmaceutical Science Research Institute, Aston University, Birmingham, UK.
Neuroreport. 2001 Jan 22;12(1):25-8. doi: 10.1097/00001756-200101220-00013.
cGMP is thought to play a role in cerebellar signalling yet its production within Purkinje cells has never been detected. In the present study, the hydrolysis of a fluorescent substrate analogue, 2'-O-anthranyloyl cyclic GMP, by type 5 phosphodiesterase was monitored within Purkinje cells in slices and in culture. Nitric oxide, either endogenously released from adjacent neurons or pharmacologically applied, accelerated the rate of hydrolysis in a manner that was dependent on soluble guanylyl cyclase, demonstrating that nitric oxide triggers cyclic GMP production in Purkinje cells, which in turn activates type 5 phosphodiesterase. We conclude that NO acts as an intercellular messenger in the cerebellar cortex and that parallel fibre terminals are a probable source of nitric oxide.
人们认为环磷酸鸟苷（cGMP）在小脑信号传导中发挥作用，但其在浦肯野细胞内的产生从未被检测到。在本研究中，在切片和培养的浦肯野细胞中监测了5型磷酸二酯酶对荧光底物类似物2'-O-邻氨基苯甲酰基环磷酸鸟苷的水解作用。一氧化氮，无论是从相邻神经元内源性释放还是通过药理学应用，都以依赖于可溶性鸟苷酸环化酶的方式加速了水解速率，表明一氧化氮触发浦肯野细胞中环磷酸鸟苷的产生，进而激活5型磷酸二酯酶。我们得出结论，一氧化氮在小脑皮质中作为一种细胞间信使起作用，并且平行纤维终末可能是一氧化氮的来源。
