Hallemeesch M M, Ten Have G A, Deutz N E
Department of Surgery, Maastricht University, The Netherlands.
Lab Anim. 2001 Jan;35(1):101-10. doi: 10.1258/0023677011911426.
A method was developed to measure metabolic fluxes across either portally-drained viscera (PDV) and liver or kidney and hindquarter (HQ) in anesthetized mice. The method includes a primed-constant infusion of ketamine-medetomidine anaesthesia to stabilize the mice for the surgical procedures. For measurement of metabolic fluxes across PDV and liver, blood sampling catheters were inserted in the carotid artery, portal vein and hepatic vein and infusion catheters in the jugular vein and mesenteric vein. For measurement of metabolic flux across kidney and HQ, blood sampling catheters were inserted in the carotid artery, renal vein and caval vein and infusion catheters in the jugular vein and abdominal aorta. 14C-PAH was infused to enable plasma flow measurement using an indicator dilution method. In addition, we developed a blood sampling procedure without waste of blood. We measured plasma flow and metabolic fluxes across PDV, liver, kidney and HQ. Mean plasma flow in post-absorptive mice was: PDV: 0.9+/-0.2, liver: 1.2+/-0.3, kidney: 1.0+/-0.1, HQ: 1.1+/-0.3 ml/10 g body weight (b.w.)/min. Significant glutamine release by the HQ and uptake of glutamine by the kidney and PDV was observed. In PDV, citrulline is produced from glutamine and is in turn used by the kidney for the production of arginine. In conclusion, the described model enables measurement of metabolic fluxes across PDV, liver, kidney and HQ in mice. The availability of such a small animal model allows the potential for measuring metabolic parameters in transgenic and knockout mice, and therefore may lead to an important refinement in metabolic research.
已开发出一种方法来测量麻醉小鼠门静脉引流内脏（PDV）与肝脏或肾脏与后肢（HQ）之间的代谢通量。该方法包括用氯胺酮 - 美托咪定进行首剂量恒速输注麻醉，以使小鼠在手术过程中保持稳定。为了测量PDV与肝脏之间的代谢通量，将采血导管插入颈动脉、门静脉和肝静脉，并将输注导管插入颈静脉和肠系膜静脉。为了测量肾脏与HQ之间的代谢通量，将采血导管插入颈动脉、肾静脉和腔静脉，并将输注导管插入颈静脉和腹主动脉。输注14C - PAH以使用指示剂稀释法测量血浆流量。此外，我们开发了一种不浪费血液的采血程序。我们测量了PDV、肝脏、肾脏和HQ的血浆流量和代谢通量。吸收后小鼠的平均血浆流量为：PDV：0.9±0.2，肝脏：1.2±0.3，肾脏：1.0±0.1，HQ：1.1±0.3 ml/10 g体重（b.w.）/分钟。观察到HQ有显著的谷氨酰胺释放，以及肾脏和PDV对谷氨酰胺的摄取。在PDV中，谷氨酰胺产生瓜氨酸，而瓜氨酸又被肾脏用于精氨酸的生成。总之，所描述的模型能够测量小鼠PDV、肝脏、肾脏和HQ之间的代谢通量。这种小动物模型的可用性使得在转基因和基因敲除小鼠中测量代谢参数成为可能，因此可能会给代谢研究带来重要的改进。
