Ten Have G A, Bost M C, Suyk-Wierts J C, van den Bogaard A E, Deutz N E
Department of Surgery, University of Limburg, Maastricht, The Netherlands.
Lab Anim. 1996 Oct;30(4):347-58. doi: 10.1258/002367796780739862.
A method was developed to measure metabolic fluxes simultaneously across the portally-drained viscera (PDV), liver, spleen, kidney and hindquarter (HQ) in the conscious pig (20-25 kg). For this purpose, sampling catheters were implanted in the abdominal artery, portal vein, hepatic vein, splenic vein, renal vein and caval vein. Further, two extra infusion catheters were implanted in the splenic vein and abdominal artery. These allow continuous infusion of para-aminohippuric acid (PAH), providing a method for estimating the plasma flow of the liver, PDV, spleen, kidney and HQ. To minimize the postoperative recovery period of the pigs, great attention was paid to the housing conditions. After a recovery period of seven days, pigs were used for experiments twice a week. During the three weeks experimental period, food intake, body temperature, weight gain, blood gas data and plasma flow were monitored. Mean plasma flow was: liver 52 +/- 6, PDV 40 +/- 5, HQ 20 +/- 2, spleen 4 +/- 1 and kidneys 15 +/- 2 ml/kg body weight/min. These data were characteristic for a pig in a conscious normal resting and unstressed state. The long-term patency rate of the sampling catheters was very high (ranging from 75% to 100%). This was probably due to the prevention of catheter-related infections using a gentamicin (20 mg/ ml), alpha-chymotrypsin (225 U/ml) solution as catheter filling. We conclude that this model enables simultaneously liver, PDV, intestine, spleen, liver, kidney and HQ flux measurement of many metabolic substances in the conscious pig.
开发了一种方法，用于在20 - 25千克的清醒猪中同时测量流经门静脉引流内脏（PDV）、肝脏、脾脏、肾脏和后肢（HQ）的代谢通量。为此，将采样导管植入腹主动脉、门静脉、肝静脉、脾静脉、肾静脉和腔静脉。此外，在脾静脉和腹主动脉中植入了另外两根输注导管。这些导管允许连续输注对氨基马尿酸（PAH），从而提供一种估算肝脏、PDV、脾脏、肾脏和HQ血浆流量的方法。为了尽量缩短猪的术后恢复期，对饲养条件给予了高度关注。经过七天的恢复期后，每周对猪进行两次实验。在为期三周的实验期内，监测食物摄入量、体温、体重增加、血气数据和血浆流量。平均血浆流量为：肝脏52±6、PDV 40±5、HQ 20±2、脾脏4±1以及肾脏15±2毫升/千克体重/分钟。这些数据是处于清醒、正常休息且无应激状态的猪的特征性数据。采样导管的长期通畅率非常高（范围为75%至100%）。这可能是由于使用庆大霉素（20毫克/毫升）、α - 糜蛋白酶（225单位/毫升）溶液作为导管填充物来预防导管相关感染。我们得出结论，该模型能够同时测量清醒猪中肝脏、PDV、肠道、脾脏、肝脏、肾脏和HQ中多种代谢物质的通量。
