Castejón O J, Castejón H V, Alvarado M V
Instituto de Investigaciones Biológicas, Facultad de Medicina, Universidad de Zulia, Apartado 526, Maracaibo, Venezuela.
Biocell. 2000 Dec;24(3):197-212.
The intracortical pathways of climbing fibers were traced in several vertebrate cerebella using light microscopy, confocal laser scanning microscopy, scanning and transmission electron microscopy. They were identified as fine fibers up to 1(micron thick, with a characteristic crossing-over bifurcation pattern. Climbing fiber collaterals were tridimensionally visualized forming thin climbing fiber glomeruli in the granular layer. Confocal laser scanning microscopy revealed three types of collateral processes at the interface between granular and Purkinje cell layers. Scanning electron microscopy showed climbing fiber retrograde collaterals in the molecular layer. Asymmetric synaptic contacts of climbing fibers with Purkinje dendritic spines and stellate neuron dendrites were characterized by transmission electron microscopy. Correlative microscopy allowed us to obtain the basic three-dimensional morphological features of climbing fibers in several vertebrates and to show with more accuracy a higher degree of lateral collateralization of these fibers within the cerebellar cortex. The correlative microscopy approach provides new views in the cerebellar cortex information processing.
利用光学显微镜、共聚焦激光扫描显微镜、扫描电子显微镜和透射电子显微镜，对几种脊椎动物小脑内的攀缘纤维皮质内通路进行了追踪。它们被识别为直径达1微米的细纤维，具有特征性的交叉分支模式。攀缘纤维侧支在颗粒层三维可视化形成薄的攀缘纤维小球。共聚焦激光扫描显微镜显示在颗粒层和浦肯野细胞层之间的界面处有三种类型的侧支过程。扫描电子显微镜显示分子层中有攀缘纤维逆行侧支。透射电子显微镜对攀缘纤维与浦肯野树突棘和星状神经元树突的不对称突触接触进行了表征。相关显微镜使我们能够获得几种脊椎动物攀缘纤维的基本三维形态特征，并更准确地显示这些纤维在小脑皮质内更高程度的侧支化。相关显微镜方法为小脑皮质信息处理提供了新的视角。
