Castejón O J, Castejón H V, Sims P
Institute of Biological Investigations, Faculty of Medicine, University of Zulia, Maracaibo, Venezuela.
J Submicrosc Cytol Pathol. 2001 Jan-Apr;33(1-2):23-32.
The cerebellar basket cells of mice, hamsters, teleost fishes and human have been studied by means of Golgi light microscopy, confocal laser scanning microscopy, scanning and transmission electron microscopy. Golgi light microscopy showed ascending dendrites toward the molecular layer and descending and transverse axonal collaterals contributing to the formation of Purkinje cell pericellular basket. Confocal laser scanning microscopy provided z-series of optodigital sections of ascending basket cell dendrites and descending axonal collaterals participating in the Purkinje cell pericellular nest and the pinceaux. Scanning electronmicrographs displayed the three-dimensional relief of Purkinje pericellular nest formed by basket cell descending and transverse axonal collaterals. Transmission electron microscopy, used as a complementary technique, showed the synaptic contacts formed by basket cell axonal collaterals on Purkinje cell soma and the axosomatic contacts on basket cells by parallel and climbing fiber endings, basket cell axonal terminals and/or Purkinje cell recurrent axonal collaterals. Pre- and postsynaptic membrane specializations were mainly found in parallel fiber axosomatic contacts on basket cells, but not in the Purkinje cell pericellular basket and in other axosomatic contacts on basket cells. These latter findings have been adscribed to a phenomenon of cerebellar synaptic plasticity related with motor learning performance. The correlative microscopy approach demonstrates the potential value of these methodologies for studying the three-dimensional aspect of short intracortical circuits in the central nervous system.
通过高尔基光学显微镜、共聚焦激光扫描显微镜、扫描电子显微镜和透射电子显微镜，对小鼠、仓鼠、硬骨鱼和人类的小脑篮状细胞进行了研究。高尔基光学显微镜显示，树突向分子层上升，轴突侧支向下和横向延伸，有助于形成浦肯野细胞周围篮状结构。共聚焦激光扫描显微镜提供了参与浦肯野细胞周围巢和夹钳的上升篮状细胞树突和下降轴突侧支的光学数字切片的z系列。扫描电子显微镜图像展示了由篮状细胞下降和横向轴突侧支形成的浦肯野细胞周围巢的三维轮廓。作为一种补充技术，透射电子显微镜显示了篮状细胞轴突侧支在浦肯野细胞胞体上形成的突触接触，以及平行纤维和攀缘纤维末梢、篮状细胞轴突终末和/或浦肯野细胞回返轴突侧支在篮状细胞上形成的轴-体接触。突触前和突触后膜特化主要见于篮状细胞上的平行纤维轴-体接触，而不见于浦肯野细胞周围篮状结构以及篮状细胞上的其他轴-体接触。后一种发现归因于与运动学习表现相关的小脑突触可塑性现象。相关显微镜方法证明了这些方法在研究中枢神经系统中短皮质内回路三维方面的潜在价值。
