白血病和肿瘤患者尿液中的腐胺、亚精胺、N-乙酰亚精胺和精胺。

Putrescine, spermidine, N-acetylspermidine and spermine in the urine of patients with leukaemias and tumors.

Tsuji M, Nakajima T, Sano I

Clin Chim Acta. 1975 Mar 10;59(2):161-7. doi: 10.1016/0009-8981(75)90024-8.

Abstract

N-Monoacetylspermidine A and B were isolated from human urine. Structures of the isolated compounds were assigned to monoacetylspermidines by analyses of the products of acid hydrolysis and elementary analyses. The identification was confirmed by comparison of the chromatographic and electrophoretic properties and infrared spectra of the isolated compounds with those of the authentic compounds. 2. The concentrations of urinary polyamines were determined by the combination of ion-exchange chromatography and high-voltage paper electrophoresis. The amount of putrescine fell in the range of 1--6;spermidine, 1--3;spermine, 3--15 and N-acetylspermidines, 2--10 nmoles/mg creatinine for both sexes and different ages. 3. The amounts of urinary polyamines were measured in patients with cancers. The remarkable increase of urinary spermine was observed in blood cancer, while in the other solid cancers the increase of urinary polyamines was not observed.

摘要

N-单乙酰亚精胺A和B从人尿中分离得到。通过对酸水解产物和元素分析，将分离出的化合物结构确定为单乙酰亚精胺。通过比较分离出的化合物与标准化合物的色谱、电泳性质及红外光谱，证实了该鉴定结果。2. 尿中多胺的浓度通过离子交换色谱和高压纸电泳相结合的方法测定。两性和不同年龄段的腐胺含量在1 - 6纳摩尔/毫克肌酐范围内；亚精胺为1 - 3；精胺为3 - 15；N-乙酰亚精胺为2 - 10。3. 对癌症患者的尿中多胺含量进行了测定。在血癌患者中观察到尿精胺显著增加，而在其他实体癌患者中未观察到尿多胺增加。