尿液中的多胺-吡哆醛席夫碱
Polyamine-pyridoxal Schiff bases in urine.
作者信息
Aigner-Held R, Campbell R A, Daves G D
出版信息
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6652-5. doi: 10.1073/pnas.76.12.6652.
Schiff bases of the diamines 1,3-diaminopropane, putrescine, and cadaverine and the polyamines spermidine and spermine with pyridoxal or pyridoxal phosphate occur in human urine, as shown by gas chromatographic/mass spectrometric selected ion-monitoring techniques. By use of synthetic standards, procedures were devised for conversion of the Schiff bases to stable derivatives amenable to gas chromatographic/mass spectrometric analysis. These procedures involve borohydride reduction of the C = N double bond, hydrolytic removal of the phosphate group, chromatographic separation from the bulk of urinary constituents, and trifluoroacetylation of polar functional groups. The levels of the polyamine-pyridoxal Schiff bases were estimated to be in the range of pmol/ml or urine.
通过气相色谱/质谱选择离子监测技术表明，1,3 - 二氨基丙烷、腐胺、尸胺等二胺以及多胺亚精胺和精胺与吡哆醛或磷酸吡哆醛形成的席夫碱存在于人类尿液中。通过使用合成标准品，设计了将席夫碱转化为适合气相色谱/质谱分析的稳定衍生物的程序。这些程序包括硼氢化物还原C = N双键、水解去除磷酸基团、从大部分尿液成分中进行色谱分离以及极性官能团的三氟乙酰化。多胺 - 吡哆醛席夫碱的水平估计在每毫升尿液pmol范围内。
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