Yoshiyama M, Urayama A, Kimochi T, Matsuo T, Pashley D H
Department of Conservative Dentistry, School of Dentistry, Tokushima University, 3-18-15, Kuramoto-cho, Tokushima 770 Japan.
Oper Dent. 2000 May-Jun;25(3):163-9.
The mechanism of resin adhesion to caries-affected dentin is still unclear. This study evaluated the interfacial morphology of two bonding systems to caries-affected dentin, coupled with the measurement of microtensile bond strengths (mu TBS). Carious human molars were prepared as previously described in Nakajima and others (1995) and were bonded with Single Bond (SB) or FluoroBond (FB) according to the manufacturer's instructions, followed by creation of AP-X composite buildups. After one day of storage in 37 degrees C water, the teeth were serially sectioned vertically into 0.8 mm slabs, trimmed to yield a 1 mm2 test area, and tested to failure in a Bencor device used in an Instron machine operated at 1 mm/min. Resin-dentin interfaces were observed with SEM before or after acid/base challenge. Bonding to normal dentin with the two bonding systems (SB and FB) showed tensile bond strengths significantly higher than those to caries-affected dentin. The moist bonding technique significantly increased bond strength of SB to normal and caries-affected dentin. SEM examination revealed that typical hybrid layer and resin tags could not be formed to caries-affected dentin. The results suggested that resin penetration may be prevented by occlusion of dentinal tubules by mineral deposits that may also impart acid-resistance to the intertubular matrix of caries-affected dentin.
树脂与龋损牙本质的黏附机制仍不明确。本研究评估了两种粘结系统与龋损牙本质的界面形态,并测量了微拉伸粘结强度(μTBS)。按照中岛等人(1995年)之前描述的方法制备龋损人类磨牙,并根据制造商的说明用单键(SB)或氟键(FB)进行粘结,随后制作AP-X复合树脂堆积。在37℃水中储存一天后,将牙齿垂直连续切成0.8mm厚的薄片,修整成1mm²的测试区域,并在Instron机器上以1mm/min的速度运行的Bencor装置中测试至破坏。在酸/碱处理前后,用扫描电子显微镜(SEM)观察树脂-牙本质界面。两种粘结系统(SB和FB)与正常牙本质的粘结显示出的拉伸粘结强度显著高于与龋损牙本质的粘结强度。湿粘结技术显著提高了SB与正常牙本质和龋损牙本质的粘结强度。SEM检查显示,无法在龋损牙本质上形成典型的混合层和树脂突。结果表明,矿物质沉积可能会堵塞牙本质小管,从而阻止树脂渗透,这些矿物质沉积也可能使龋损牙本质的管间基质具有耐酸性。