McEwen J G, Taylor J W, Carter D, Xu J, Felipe M S, Vilgalys R, Mitchell T G, Kasuga T, White T, Bui T, Soares C M
Molecular Biology Unit, Corporatión para Investigaciones Biológicas, Medellín, Colombia.
Med Mycol. 2000;38 Suppl 1:189-97.
In this Round Table, the application of several methods of molecular typing were discussed in reference to four important pathogenic fungi: Coccidioides immitis, Histoplasma capsulatum, Candida albicans and Paracoccidioides brasiliensis. Among the different methods the following were discussed: restriction fragment length polymorphisms (RFLP), single nucleotide polymorphisms, random amplified polymorphic DNA (RAPD), polymerase chain reaction (PCR)-RFLP and microsatellites. By means of these methods, several important biological questions related to speciation, mode of reproduction and population genetics could be approached. The basic information obtained from this approach has implications in the understanding of these pathogenic fungi in relation to their behavior and the development of pathogenic features, such as resistance to antimicrobials and virulence factors used for colonization of mammalian hosts. The knowledge obtained from these studies could also be used for the development of innovative diagnostic methods, as well as for novel therapeutic approaches and production of vaccines.
在本次圆桌会议中，针对四种重要的致病真菌：粗球孢子菌、荚膜组织胞浆菌、白色念珠菌和巴西副球孢子菌，讨论了几种分子分型方法的应用。在不同方法中，讨论了以下几种：限制性片段长度多态性（RFLP）、单核苷酸多态性、随机扩增多态性DNA（RAPD）、聚合酶链反应（PCR）-RFLP和微卫星。通过这些方法，可以探讨与物种形成、繁殖方式和群体遗传学相关的几个重要生物学问题。从这种方法获得的基本信息对于理解这些致病真菌的行为及其致病特征的发展具有重要意义，例如对抗菌药物的抗性和用于在哺乳动物宿主中定殖的毒力因子。从这些研究中获得的知识还可用于开发创新的诊断方法，以及新型治疗方法和疫苗生产。
