Dobrowolska Anita, Bojarski Lukasz, Staczek Paweł
Zakład Genetyki Drobnoustrojów, Instytut Mikrobiologii i Immunologii, Uniwersytet Lódzki, ul. Banacha 12/16, 90-237 Lódź.
Wiad Parazytol. 2002;48(3):241-55.
In this paper we reviewed the latest literature on molecular techniques used in diagnosis and epidemiology of infections caused by pathogenic fungi. Traditional methods used for the identification and typing of medically relevant fungi include morphological and biochemical analysis. These methods are time-consuming and base on phenotypic features what makes them unreliable. We described the usefulness in mycological studies of fast and very sensitive molecular methods which rely on PCR and hybridization techniques.
在本文中,我们回顾了关于用于致病性真菌引起的感染的诊断和流行病学研究的分子技术的最新文献。用于医学相关真菌鉴定和分型的传统方法包括形态学和生化分析。这些方法耗时且基于表型特征,这使得它们不可靠。我们描述了依赖于PCR和杂交技术的快速且非常灵敏的分子方法在真菌学研究中的实用性。