Ilori M O, Amund D I
Department of Botany and Microbiology, University of Lagos, Akoka-Yaba, Nigeria.
Z Naturforsch C J Biosci. 2000 Nov-Dec;55(11-12):890-7. doi: 10.1515/znc-2000-11-1208.
Four bacteria, identified as Pseudomonas aeruginosa, Alcaligenes eutrophus, Bacillus subtilis and Micrococcus luteus were isolated from crude oil polluted soils using anthracene as the sole carbon and energy source. All the organisms utilized n-hexadecane, n-tetradecane, diesel oil, engine oil and naphthalene as sole carbon sources. None could utilize hexane, cycloheptane, xylene, benzene, toluene, phenol, fluoranthene,and kerosene as carbon sources. Highest cell density obtained with 0.1% (w/v) anthracene were 4.5 x 10(7) (cfu/ml), 8.6 x 10(6) (cfu/ml), 5.4 x 10(6) and 2.4 x 10(6) (cfu/ml) respectively, for P. aeruginosa, A. eutrophus, B. subtilis and M. luteus after 30 days incubation. Growth of the organisms on a Nigerian crude oil resulted in a residual oil concentration of 22.2%, 33.3%, 39.3%, 44% and 91.7% respectively, for P. aeruginosa, A. eutrophus, B. subtilis, M. luteus and the noninoculated control on the 14 th day. Ring fission enzymes of the meta pathway were detected in induced cells of P. aeruginosa and A. eutrophus while ortho pathway enzymes were detected in B. subtilis and M. luteus. P. aeruginosa and A. eutrophus had specific catechol-2,3-dioxygenase activities of 3.8 +/- 0.183 and 0.64 +/- 0.032 micromol/min x mg protein respectively while catechol-1,2-dioxygenase activities of 1.95 +/- 0.029 and 1.89 +/- 0.026 micromol/min x mg protein were detected in B. subtilis and M. luteus respectively. This work, highlights the capability of these unreported tropical strains of A. eutrophus, B. subtilis and M. luteus as anthracene degraders.
以蒽作为唯一碳源和能源，从原油污染土壤中分离出四种细菌，分别鉴定为铜绿假单胞菌、嗜碱产碱杆菌、枯草芽孢杆菌和藤黄微球菌。所有这些微生物都能利用正十六烷、正十四烷、柴油、机油和萘作为唯一碳源。但无一能利用己烷、环庚烷、二甲苯、苯、甲苯、苯酚、荧蒽和煤油作为碳源。在含有0.1%（w/v）蒽的培养基中，培养30天后，铜绿假单胞菌、嗜碱产碱杆菌、枯草芽孢杆菌和藤黄微球菌获得的最高细胞密度分别为4.5×10⁷（cfu/ml）、8.6×10⁶（cfu/ml）、5.4×10⁶和2.4×10⁶（cfu/ml）。在尼日利亚原油上培养这些微生物，在第14天时，铜绿假单胞菌、嗜碱产碱杆菌、枯草芽孢杆菌、藤黄微球菌和未接种对照的残留油浓度分别为22.2%、33.3%、39.3%、44%和91.7%。在铜绿假单胞菌和嗜碱产碱杆菌的诱导细胞中检测到间位途径的环裂解酶，而在枯草芽孢杆菌和藤黄微球菌中检测到邻位途径的酶。铜绿假单胞菌和嗜碱产碱杆菌的特异性儿茶酚-2,3-双加氧酶活性分别为3.8±0.183和0.64±0.032微摩尔/分钟×毫克蛋白，而在枯草芽孢杆菌和藤黄微球菌中分别检测到儿茶酚-1,2-双加氧酶活性为1.95±0.029和1.89±0.026微摩尔/分钟×毫克蛋白。这项工作突出了这些未报道的热带嗜碱产碱杆菌、枯草芽孢杆菌和藤黄微球菌菌株作为蒽降解菌的能力。
