Department of Microbiology, University of Port Hacourt, Port Hacourt, Rivers State, Nigeria.
Bull Environ Contam Toxicol. 2012 Jun;88(6):1014-9. doi: 10.1007/s00128-012-0598-7. Epub 2012 Mar 29.
Polycyclic aromatic hydrocarbon (PAH)-degrading bacteria were isolated from aviation fuel contaminated soil at Inua Eyet Ikot in Ibeno, Nigeria. PAH-degrading bacteria in the contaminated soil were isolated by enrichment culture technique. Isolates with high PAH degrading potential characterized by their extensive growth on PAH-supplemented minimal salt medium were screened for their naphthalene, phenanthrene and chrysene degradability. The screening medium which contained selected PAHs as the sole source of carbon and energy showed that Micrococcus varians AFS-2, Pseudomonas putida AFS-3 and Alcaligenes faecalis AFS-5 exhibited a concentration-dependent growth in all the PAH-compounds tested. There were visible changes in the color of growth medium suggesting the production of different metabolites. Their acclimation to different PAH substrates was also evident as A. faecalis AFS-5 isolated from chrysene grew well on other less complex aromatic compounds. The isolate exhibited best growth (0.44 OD(600)) when exposed to 10 ppm of chrysene for 5 days and could utilize up to 90 ppm of chrysene. This isolate and others with strong PAH-degrading potentials are recommended for bioremediation of PAHs in aviation fuel-contaminated sites in the tropics.
从尼日利亚伊本诺的因瓦埃耶伊科特航空燃料污染土壤中分离出了多环芳烃（PAH）降解菌。采用富集培养技术从污染土壤中分离出 PAH 降解菌。通过在添加 PAH 的最小盐培养基上广泛生长的特性，对具有高 PAH 降解潜力的分离物进行了萘、菲和芘的降解能力筛选。筛选培养基以选定的 PAH 为唯一的碳源和能源，表明微球菌 AFS-2、假单胞菌 AFS-3 和粪产碱杆菌 AFS-5 均能在所有测试的 PAH 化合物中表现出浓度依赖性生长。生长培养基的颜色发生了明显变化，表明产生了不同的代谢物。它们对不同 PAH 基质的适应能力也很明显，因为从芘中分离出的粪产碱杆菌 AFS-5 可以在其他较简单的芳香族化合物上良好生长。该分离物在暴露于 10ppm 芘 5 天时表现出最佳生长（0.44 OD（600）），并可以利用高达 90ppm 的芘。建议将该分离物和其他具有较强 PAH 降解潜力的分离物用于热带地区航空燃料污染场地的 PAH 生物修复。
