Batalova F M, Stepanova I S, Gogoliubov D S
Institute of Cytology RAS, St. Petersburg.
Tsitologiia. 2000;42(11):1037-47.
The absence of nucleoli and rRNA synthesis in oocyte nuclei of the scorpion fly, Panorpa communis, was demonstrated using hybridization in situ. The immunocytochemical characteristics of a single large nuclear body of P. communis oocytes has been done which enabled us to recognize this structure as a Cajal body (CB). A marker of CBs coilin was detected in CBs of P. communis oocytes in addition to Sm-epitope of small nuclear RNPs (snRNPs), trimethylguanosine cap of snRNAs, and non-snRNP splicing factor, SC35. Besides, inactive hyperphosphorylated and nonphosphorylated forms of RNA polymerase II were also found in CBs. We put forward a suposition on possible roles of CBs in P. communis oocytes. On the one hand, CBs take part in the storage of RNA transcription and processing components to be used later during embryogenesis. On the other hand, they provide the primary assembly and redistribution of pre-mRNA transcription and processing components of the oocyte itself.
利用原位杂交技术证实了蝎蛉（Panorpa communis）卵母细胞核中核仁及核糖体RNA（rRNA）合成的缺失。对普通蝎蛉卵母细胞中单个大的核体进行了免疫细胞化学特征分析，并据此将该结构识别为卡哈尔体（CB）。除了小分子核核糖核蛋白（snRNP）的Sm表位、snRNA的三甲基鸟苷帽和非snRNP剪接因子SC35外，在普通蝎蛉卵母细胞的CB中还检测到了CB的标志物卷曲螺旋蛋白。此外，在CB中还发现了RNA聚合酶II的无活性高磷酸化和非磷酸化形式。我们对普通蝎蛉卵母细胞中CB的可能作用提出了一种推测。一方面，CB参与RNA转录和加工成分的储存，以便在胚胎发育后期使用。另一方面，它们为卵母细胞自身的前体mRNA转录和加工成分提供初步组装和重新分布。
