Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA.
Arch Virol. 2010 Mar;155(3):441-3. doi: 10.1007/s00705-009-0586-3. Epub 2010 Feb 18.
Plants of Triteleia hyacinthina, Triteleia ixioides Starlight, and Triteleia laxa Corina with severe mosaic and yellow vein-banding were found to be infected with a potyvirus. The 3'-terminal region of the virus was amplified by RT-PCR from total RNA using a potyvirus-specific degenerate primer (poty5P: 5' GGN AAY AAY AGY GGN CAR CC 3') and an oligo-dTprimer. The sequence generated included the 3'-NIb protein coding region (680 nucleotides), the entire coat protein coding region (840 nucleotides), and 3'-untranslated region (UTR) (253 nucleotides). Amino acid identity of the whole CP between the triteleia virus and potyvirus member ranged from 54% Apium virus Y (ApVY) to 67% Auraujia mosaic virus (ArjMV) and Twisted-stalk chlorotic streak virus (TSCSV) and the core ranged from 59% (ApVY) to 75% (ArjMV). The 3-UTR showed no significant homology with other known potyviruses. Phylogenetic relationships suggest this triteleia virus is a new member of the Potyvirus genus and the name of "Triteleia mosaic virus" (TriMV) is proposed. This is the first report of a potyvirus infecting triteleia.
发现严重花叶和黄脉带的翠雀花属植物（Triteleia hyacinthina、Triteleia ixioides Starlight 和 Triteleia laxa Corina）感染了马铃薯 Y 病毒属病毒。使用马铃薯 Y 病毒属特异性简并引物（poty5P：5' GGN AAY AAY AGY GGN CAR CC 3'）和 oligo-dT 引物，通过 RT-PCR 从总 RNA 扩增病毒的 3'末端区。生成的序列包括 3'-NIb 蛋白编码区（680 个核苷酸）、整个外壳蛋白编码区（840 个核苷酸）和 3'-UTR（253 个核苷酸）。该病毒与马铃薯 Y 病毒属成员的整个 CP 的氨基酸同一性范围为 54% Apium virus Y (ApVY) 至 67% Auraujia mosaic virus (ArjMV) 和 Twisted-stalk chlorotic streak virus (TSCSV)，核心区为 59%（ApVY）至 75%（ArjMV）。3'-UTR 与其他已知的马铃薯 Y 病毒属病毒没有显著同源性。系统发育关系表明，该翠雀花病毒是马铃薯 Y 病毒属的一个新成员，建议将其命名为“翠雀花花叶病毒”（TriMV）。这是首例感染翠雀花的马铃薯 Y 病毒属病毒报告。
