散发性同步性结直肠癌中的错配修复缺陷

Mismatch repair deficiency in sporadic synchronous colorectal cancer.

作者信息

Brueckl W M, Limmert T, Brabletz T, Guenther K, Jung A, Hermann K, Wiest G H, Kirchner T, Hohenberger W, Hahn E G, Wein A

机构信息

Department of Internal Medicine I, University of Erlangen-Nuremberg, Krankenhausstr, 12, 91054 Erlangen, Germany.

出版信息

Anticancer Res. 2000 Nov-Dec;20(6C):4727-32.

PMID:11205208

Abstract

BACKGROUND

Hereditary non-polyposis colorectal cancer (HNPCC) patients frequently develop synchronous colorectal cancer (SCRC) which also occurs sporadically in other patients. Recent studies on microsatellite instability (MSI) in sporadic SCRC diverge completely in their findings (0%-100%). In the present study MSI and mismatch repair (MMR) proteins were evaluated according to standardised criteria (exclusion of a family history, MSI analysed according to NCI recommendations)

METHODS

Paraffin embedded sections of SCRC of 30 patients were evaluated for MSI and the loss of protein expression of hMLH1 and hMSH2.

RESULTS

3 out of 30 (10%) patients exhibited MSI-H which 5 out of 30 (17%) showed MSI-L. Loss of protein expression of either hMLH1 or hMSH2 was found in all cases of MSI-H and none of the MSI-L cancers.

CONCLUSION

MSI is found in sporadic cases of SCRC to about the same extent as it is mentioned in the literature on sporadic single colorectal cancers. Immunohistochemistry with mismatch repair proteins could be used as a pre-screening for MMR deficiency in sporadic SCRC.

摘要

背景

遗传性非息肉病性结直肠癌（HNPCC）患者常发生同时性结直肠癌（SCRC），散发性SCRC也见于其他患者。近期关于散发性SCRC微卫星不稳定性（MSI）的研究结果完全不同（0%-100%）。在本研究中，根据标准化标准（排除家族史，按照美国国立癌症研究所（NCI）建议分析MSI）对MSI和错配修复（MMR）蛋白进行评估。