干扰素-γ可增加人胶质母细胞瘤细胞系中白细胞介素-6的产生。
Interferon-gamma increases IL-6 production in human glioblastoma cell lines.
作者信息
Hotfilder M, Knupfer H, Mohlenkamp G, Pennekamp P, Knupfers M, Van Gool S, Wolff J E
机构信息
University Münster, Department of Pediatric Hematology/Oncology, Domagkstr. 3, Münster, Germany.
出版信息
Anticancer Res. 2000 Nov-Dec;20(6B):4445-50.
BACKGROUND
Various immunotherapeutical approaches are presently evaluated for their efficacy to eradicate glioma cells. Complicating the concepts, these tumors secrete cytokines which modulate the immune response.
MATERIALS & METHODS: We analyzed the influence of interferon gamma (IFN-gamma) on the cytokine production and IFN-gamma receptor expression in T98G and U87-MG human glioma cells.
RESULTS
The IFN-gamma receptors were own-regulated after IFN-gamma treatment. Secretion of interleukin-6 (IL-6) protein was elevated by factors of 6.4 in T98G cells and 5.2 in U87-MG. Other cytokines were increased as well, but less constantly: IL-8, and VEGF were elevated significantly only in T98G, but not in U87-MG. Increases of IL-1 beta, IL-1 alpha and TGF beta-2 were only detectable at the mRNA level. TNF was not detectable in any of the cell lines, and TGF-beta, alpha FGF and HG were not influenced by IFN-gamma.
CONCLUSION
IL-6 produced by glioma cells in response to IFN-gamma might support immune eradication of glioma cells.
背景
目前正在评估各种免疫治疗方法根除胶质瘤细胞的疗效。使情况复杂化的是，这些肿瘤会分泌调节免疫反应的细胞因子。
材料与方法
我们分析了干扰素γ（IFN-γ）对T98G和U87-MG人胶质瘤细胞中细胞因子产生及IFN-γ受体表达的影响。
结果
IFN-γ处理后，IFN-γ受体呈自身调节。白细胞介素-6（IL-6）蛋白的分泌在T98G细胞中升高了6.4倍，在U87-MG细胞中升高了5.2倍。其他细胞因子也有所增加，但不太稳定：IL-8和血管内皮生长因子（VEGF）仅在T98G细胞中显著升高，而在U87-MG细胞中未升高。IL-1β、IL-1α和转化生长因子β2（TGF-β2）的增加仅在mRNA水平上可检测到。在任何细胞系中均未检测到肿瘤坏死因子（TNF），且TGF-β、α成纤维细胞生长因子（αFGF）和肝细胞生长因子（HG）不受IFN-γ影响。
结论
胶质瘤细胞对IFN-γ产生的IL-6可能有助于免疫根除胶质瘤细胞。
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