Cardillo M R, Sale P, Di Silverio F
Department of Experimental Medicine and Pathology (Section of Uropathology), University La Sapienza, Rome, Italy.
Anticancer Res. 2000 Nov-Dec;20(6B):4579-83.
The progression of transitional-cell carcinomas of the bladder is associated with changes in general and local immune status. To understand the factors involved in the progression of transitional cell carcinoma and in the maintenance of an efficient anti-tumoural response, in this study we investigated by immunohistochemistry expression of HSP-90, IL-6 and IL-10 proteins in 56 surgical specimens obtained from superficial and deeply invasive bladder carcinomas. Of the 56 bladder carcinoma 52 (92.9%) expressed HSP-90, 48 (85.7%) IL-6 and 45 (80.3%) IL-10. High-grade and muscle-invasive tumours contained significantly higher levels of HSP-90 and IL-6 antibodies than low-grade and superficial tumours (p < 0.05). Linear regression showed a significant correlation between HSP-90 and IL-10 (p = 0.022) but not between HSP-90 and IL-6, or IL-6 and IL-10 expression. The variable quantities of HSP-90, IL-6 and IL-10 in the high-grade bladder carcinomas studied suggest that these proteins have independent functional roles and may be the immunogenic targets for an anti-tumoural response.
膀胱移行细胞癌的进展与全身和局部免疫状态的变化相关。为了解参与移行细胞癌进展及维持有效抗肿瘤反应的因素，在本研究中，我们通过免疫组织化学方法调查了取自浅表性和浸润性膀胱癌的56份手术标本中HSP-90、IL-6和IL-10蛋白的表达情况。在56例膀胱癌中，52例（92.9%）表达HSP-90，48例（85.7%）表达IL-6，45例（80.3%）表达IL-10。高级别和肌层浸润性肿瘤所含HSP-90和IL-6抗体水平显著高于低级别和浅表性肿瘤（p < 0.05）。线性回归显示HSP-90与IL-10之间存在显著相关性（p = 0.022），但HSP-90与IL-6之间以及IL-6与IL-10表达之间无相关性。在研究的高级别膀胱癌中，HSP-90、IL-6和IL-10数量各异，提示这些蛋白具有独立的功能作用，可能是抗肿瘤反应的免疫原性靶点。
