Cardillo Maria Rosaria, Ippoliti Flora
Department of Experimental Medicine and Pathology, Section of Pathologic Anatomy, Service of Uropathology, University "La Sapienza", Policlinico Umberto I, 324 Viale Regina Elena, 00161 Rome, Italy.
Anticancer Res. 2006 Sep-Oct;26(5A):3409-16.
The interleukins (IL)-6 and -10 and heat shock proteins (HSP) have an important role in the host-tumor interaction and in tumor bulk. HSP-90 may have a regulatory role in cytokine biosynthesis and prognostic value in some tumors. To define the role of IL-6, IL-10 and HSP-90 in prostate cancer progression the immunohistochemical expressions of these proteins were analyzed in 168 prostatic carcinomas. IL-6, IL-10 and HSP-90 immunoreactivity was higher in prostatic carcinoma (CaP) and intra-epithelial prostatic neoplasia (PIN) than in normal prostatic tissue (NAP) adjacent to neoplasia. In the epithelium, IL-6, IL-10 and HSP-90 expressions increased from NAP to PIN to CaP. In the stroma, IL-6 and IL-10 expressions decreased significantly from NAP to PIN to CaP (p < 0.01 by Chi-square test), while HSP-90 expression increased. In the epithelium of PIN and CaP, IL-6 immunoreactivity was significantly lower than IL-10 and HSP-90. In neoplastic acini HSP-90 levels were significantly higher than those of IL-6 and IL-10 (p < 0.01 by Chi-square test). In the stroma of NAP and PIN, but not of CaP, HSP-90 immunoreactivity was significantly lower than that of IL-6 and IL-10 (p < 0.01). Our results indicate that the IL-6 and IL-10 cytokine balance differs in pathological and normal prostate, thus suggesting that certain cytokines are specific to the neoplastic prostate. Changes in the expressions of IL-6, IL-10 and HSP-90 in human prostate carcinoma samples could be used as a prognostic marker of disease progression.
白细胞介素（IL）-6、-10和热休克蛋白（HSP）在宿主与肿瘤的相互作用以及肿瘤体积方面发挥着重要作用。HSP-90可能在细胞因子生物合成中具有调节作用，并且在某些肿瘤中具有预后价值。为了明确IL-6、IL-10和HSP-90在前列腺癌进展中的作用，对168例前列腺癌组织中这些蛋白的免疫组化表达进行了分析。前列腺癌（CaP）和前列腺上皮内瘤变（PIN）中IL-6、IL-10和HSP-90的免疫反应性高于肿瘤旁正常前列腺组织（NAP）。在前列腺上皮中，IL-6、IL-10和HSP-90的表达从NAP到PIN再到CaP逐渐增加。在前列腺基质中，IL-6和IL-10的表达从NAP到PIN再到CaP显著降低（卡方检验，p<0.01），而HSP-90的表达增加。在PIN和CaP的上皮中，IL-6的免疫反应性显著低于IL-10和HSP-90。在肿瘤腺泡中，HSP-90水平显著高于IL-6和IL-10（卡方检验，p<0.01）。在NAP和PIN的基质中，而非CaP的基质中，HSP-90的免疫反应性显著低于IL-6和IL-10（p<0.01）。我们的结果表明，IL-6和IL-10细胞因子平衡在病理状态和正常前列腺组织中存在差异，这表明某些细胞因子是前列腺肿瘤所特有的。人前列腺癌样本中IL-6、IL-10和HSP-90表达的变化可作为疾病进展的预后标志物。
