Department of Obstetrics and Gynaecology, Affiliated Hospital of Weifang Medical University, 2428 Yuhe Road, Weifang, 261042, China.
Department of Obstetrics and Gynaecology, Qilu Hospital, Shandong University, Jinan, China.
Virol J. 2021 Jan 6;18(1):6. doi: 10.1186/s12985-020-01474-z.
High-risk human papillomavirus (HR-HPV) load is thought to be influenced by many factors, and the relationship between viral load and the degree of cervical lesion is controversial. This study explored the possible influencing factors of HR-HPV viral load in the uterine cervix.
A total of 605 women who needed colposcopic evaluation for abnormal cervical screening at the Affiliated Hospital of Weifang Medical University, China, between November 2017 and September 2018 were enrolled. Cervical specimens were collected from the endo- and ectocervix separately using two different cervical brushes. The hybrid capture II test was used to measure HR-HPV load. Age, histological severity, number of viral types, and area and location of cervical lesions were recorded. The correlations between viral load and influencing factors were analysed using univariate and multivariate analyses.
HR-HPV load was positively correlated with age, histological severity, multiple HPV types and area of cervical lesions (P < 0.05). Viral load with the combination of endo- and ectocervical sampling was significantly higher than simple endocervical sampling (P < 0.001). Multivariate analysis showed that age, multiple HPV types and area of cervical lesions were independent factors for HR-HPV load with a combination of endo- and ectocervical sampling (P < 0.05). However, only age and area of cervical lesions were independent factors for viral load with simple endocervical sampling (P < 0.05). No significant association was found between viral load and lesion severity in multivariate analysis (P > 0.05).
HR-HPV load is influenced by age, histological severity, multiple viral types, area of cervical lesion and sampling methods. Age and area of cervical lesions are independent factors for viral load.
高危型人乳头瘤病毒（HR-HPV）载量被认为受多种因素影响，而病毒载量与宫颈病变程度的关系尚存在争议。本研究旨在探讨宫颈 HR-HPV 载量的可能影响因素。
选取 2017 年 11 月至 2018 年 9 月在潍坊医学院附属医院因宫颈筛查异常行阴道镜检查的 605 例女性为研究对象。使用两种不同的宫颈刷分别采集宫颈内口和外口的宫颈标本，采用杂交捕获二代法检测 HR-HPV 载量，记录年龄、组织学严重程度、HPV 型别数量及宫颈病变的部位和范围。采用单因素和多因素分析方法分析病毒载量与影响因素的相关性。
HR-HPV 载量与年龄、组织学严重程度、HPV 多重感染及宫颈病变面积呈正相关（P<0.05）。内、外口联合取材的 HR-HPV 载量明显高于单纯内口取材（P<0.001）。多因素分析显示，内、外口联合取材的 HR-HPV 载量与年龄、HPV 多重感染及宫颈病变面积相关（P<0.05），而单纯内口取材的 HR-HPV 载量仅与年龄和宫颈病变面积相关（P<0.05）。多因素分析显示，病变严重程度与 HR-HPV 载量无明显相关性（P>0.05）。
HR-HPV 载量受年龄、组织学严重程度、HPV 多重感染、宫颈病变面积及取材方法影响，年龄和宫颈病变面积是 HR-HPV 载量的独立影响因素。
