Section of Microbiology, Interdisciplinary Department of Medicine (DIM),School of Medicine, University of Bari 'Aldo Moro',Bari,Italy.
UOC Microbiology and Virology, Azienda Ospedaliera-Universitaria Policlinico of Bari,Bari,Italy.
Epidemiol Infect. 2019 Jan;147:e132. doi: 10.1017/S0950268818003539.
The aim of this study was to determine the frequency of multiple type human papillomavirus (HPV) infections, and whether any types are involved in multiple HPV-type infections (mHPV) more or less frequently than expected. From January 2012 to February 2018, 2848 cervico-vaginal swabs were analysed in the UOC Microbiology and Virology of Policlinico of Bari, Italy. HPV DNA detection was performed using initially nested-polymerase chain reaction (PCR) and subsequently multiplex real-time PCR assay. 1357/2848 samples (47.65%) were HPV DNA positive and 694/1357 (51.14%) showed mHPVs. The median number of mHPVs was 2 (interquartile range: 2-3). HPV-types more frequently detected were 42 (9.97%), 16 (8.92%), 53 (7.23%) and 31 (7.16%). Each detected HPV-type was involved in mHPVs in more than 50% of cases. Statistical analysis showed significant associations for all HPV-types except for 33, 43, 51, 58 and 82 HPV-types. The major number of significant pairwise associations were detected for the types 42 and 70. Only positive associations were detected. Further data are necessary to evaluate the clinical impact of the single combinations.
本研究旨在确定多种类型人乳头瘤病毒（HPV）感染的频率，以及是否有任何类型的 HPV 更频繁或较少地参与多种 HPV 型感染（mHPV）。 2012 年 1 月至 2018 年 2 月，对意大利巴里综合医院妇产科微生物学和病毒学系的 2848 例宫颈阴道拭子进行了分析。HPV DNA 的检测最初使用巢式聚合酶链反应（PCR），随后使用多重实时 PCR 检测。2848 例样本中，1357 例（47.65%）HPV DNA 阳性，694 例（51.14%）显示 mHPV。mHPV 的中位数为 2 个（四分位间距：2-3）。检测到的 HPV 型别中，42 型（9.97%）、16 型（8.92%）、53 型（7.23%）和 31 型（7.16%）更为常见。每种 HPV 型别都有超过 50%的病例涉及 mHPV。除 33、43、51、58 和 82 型 HPV 外，对所有 HPV 型别的统计分析均显示存在显著相关性。在检测到的 HPV 型别中，42 型和 70 型之间的显著关联数量最多。仅检测到阳性关联。需要进一步的数据来评估单个组合的临床影响。
