Jung K, Lein M, Brux B, Sinha P, Schnorr D, Loening S A
Department of Urology, University Hospital Charité, Humboldt University Berlin, Germany.
Clin Chem Lab Med. 2000 Dec;38(12):1271-5. doi: 10.1515/CCLM.2000.200.
The effect of sample collection, storage conditions (time and temperature), and freeze-thaw cycles on the stability of free prostate-specific antigen (fPSA), PSA complexed with alpha1-antichymotrypsin (ACT-PSA), and total PSA (tPSA) in serum was studied. The analytes were quantified using immunoassays for tPSA and fPSA on the Elecsys system 2010 and a research assay for ACT-PSA on the ES system (Roche Diagnostics). The stability of the analytes was calculated as percentages of the values measured in samples 1 h after blood collection. When the samples were stored at 37 degrees C, at room temperature or at 4 degrees C, the stability of ACT-PSA was less impaired than that of fPSA. To avoid erroneous results in the determination of PSA isoforms and their corresponding ratios, serum samples should be preserved at 4 degrees C when the analysis is performed within 8 h after blood collection, or they should be stored at -80 degrees C if the analysis is not feasible during that period.
研究了样本采集、储存条件（时间和温度）以及冻融循环对血清中游离前列腺特异性抗原（fPSA）、与α1-抗糜蛋白酶复合的PSA（ACT-PSA）和总PSA（tPSA）稳定性的影响。使用Elecsys 2010系统上的tPSA和fPSA免疫测定法以及ES系统（罗氏诊断）上的ACT-PSA研究测定法对分析物进行定量。分析物的稳定性以采血后1小时样本中测得值的百分比计算。当样本在37℃、室温或4℃储存时，ACT-PSA的稳定性受损程度低于fPSA。为避免在PSA异构体及其相应比率的测定中出现错误结果，采血后8小时内进行分析时，血清样本应保存在4℃，如果在此期间无法进行分析，则应保存在-80℃。
