A posteriori processing of spatiotemporal digital microholograms.

In an accompanying paper [G. Indebetouw and P. Klysubun, J. Opt. Soc. Am. A 18, 319 (2001)], the theoretical background of a spatiotemporal digital microholographic method was described, and some experimental results were presented. Here the usefulness of the method for microholographic imaging of biological specimens such as cells is demonstrated. The vast possibility of a posteriori processing of the microholograms is discussed. Dark-field, phase-contrast, and interference-contrast images, as well as quantitative phase maps, all obtained a posteriori from the same microhologram, are illustrated as examples.