Shaw S L, Salmon E D, Quatrano R S
Department of Biology, University of North Carolina at Chapel Hill 27599-3280, USA.
Biotechniques. 1995 Dec;19(6):946-55.
In this report, we describe a relatively inexpensive method for acquiring, storing and processing light microscope images that combines the advantages of video technology with the powerful medium now termed digital photography. Digital photography refers to the recording of images as digital files that are stored, manipulated and displayed using a computer. This report details the use of a gated video-rate charge-coupled device (CCD) camera and a frame grabber board for capturing 256 gray-level digital images from the light microscope. This camera gives high-resolution bright-field, phase contrast and differential interference contrast (DIC) images but, also, with gated on-chip integration, has the capability to record low-light level fluorescent images. The basic components of the digital photography system are described, and examples are presented of fluorescence and bright-field micrographs. Digital processing of images to remove noise, to enhance contrast and to prepare figures for printing is discussed.
在本报告中，我们描述了一种相对廉价的获取、存储和处理光学显微镜图像的方法，该方法结合了视频技术的优点与如今被称为数码摄影的强大媒介。数码摄影是指将图像记录为数字文件，这些文件可使用计算机进行存储、处理和显示。本报告详细介绍了使用门控视频速率电荷耦合器件（CCD）相机和图像采集卡从光学显微镜捕获256级灰度数字图像。该相机可提供高分辨率的明场、相差和微分干涉相差（DIC）图像，而且通过门控片上积分，还能够记录低光水平的荧光图像。文中描述了数码摄影系统的基本组件，并给出了荧光和明场显微照片的示例。还讨论了对图像进行数字处理以去除噪声、增强对比度以及为打印准备图形的方法。
