Nishiya K, Chikazawa H, Hashimoto K, Miyawaki S
Second Department of Internal Medicine, Kochi Medical School, Nankoku City, Japan.
Clin Rheumatol. 1999;18(3):268-71. doi: 10.1007/s100670050100.
The incidence, specificity and clinical significance of positivity for serum antineutrophil cytoplasmic antibody (ANCA) was investigated in 60 patients with primary Sjögren's syndrome (SjS). The indirect immunofluorescence (IIF) technique and an enzyme-linked immunosorbent assay (ELISA) were used to measure ANCA. Purified myeloperoxidase (MPO), lactoferrin (LF), cathepsin-G (CTG) and elastase (HLE) served as ANCA antigens for the ELISA. Ten (16.7%) of the 60 SjS patients showed positivity by IIF for perinuclear, but not cytoplasmic, ANCA. Four of the 60 sera were shown to be positive for LF, four for MPO, 0 for CTG and 0 for HLE by ELISA. There was no correlation between ANCA positivity and clinical features. ANCA in patients with SjS might be an epiphenomenon of polyclonal B-cell activation.
对60例原发性干燥综合征（SjS）患者血清抗中性粒细胞胞浆抗体（ANCA）阳性的发生率、特异性及临床意义进行了研究。采用间接免疫荧光（IIF）技术和酶联免疫吸附测定（ELISA）检测ANCA。纯化的髓过氧化物酶（MPO）、乳铁蛋白（LF）、组织蛋白酶G（CTG）和弹性蛋白酶（HLE）用作ELISA的ANCA抗原。60例SjS患者中有10例（16.7%）通过IIF显示核周而非胞浆ANCA阳性。ELISA显示60份血清中有4份LF阳性，4份MPO阳性，CTG为0份阳性，HLE为0份阳性。ANCA阳性与临床特征之间无相关性。SjS患者的ANCA可能是多克隆B细胞活化的一种附带现象。
