Hsu P N, Wolf Bryant P, Sutkowski N, McLellan B, Ploegh H L, Huber B T
Program in Immunology, Department of Pathology, Tufts University School of Medicine, Boston, MA 02111, USA.
J Immunol. 2001 Mar 1;166(5):3309-14. doi: 10.4049/jimmunol.166.5.3309.
Mouse mammary tumor viruses encode superantigens that interact with MHC class II proteins and stimulate T cells. We show here that presentation of mouse mammary tumor virus superantigen does not require DM. Furthermore, we have identified a strong class II peptide binding motif in the Mtv-7 superantigen, and we show that this motif is necessary for association with class II molecules in in vitro translation and in vivo functional assays. Our results suggest that endogenously synthesized viral superantigen can bind to MHC class II heterodimers during biosynthesis in the endoplasmic reticulum in a manner analogous to that used by the class II-associated invariant chain.
小鼠乳腺肿瘤病毒编码与MHC II类蛋白相互作用并刺激T细胞的超抗原。我们在此表明，小鼠乳腺肿瘤病毒超抗原的呈递不需要DM。此外，我们在Mtv-7超抗原中鉴定出一个强II类肽结合基序，并且我们表明该基序对于在体外翻译和体内功能测定中与II类分子结合是必需的。我们的结果表明，内源性合成的病毒超抗原能够在内质网生物合成过程中以类似于II类相关恒定链所采用的方式与MHC II类异二聚体结合。
