胆囊收缩素-A受体在未成熟大鼠脑腹内侧下丘脑神经元上的功能性表达。

Functional expression of cholecystokinin-A receptor on ventromedial hypothalamic neurons in the immature rat brain.

作者信息

Sorimachi M, Yamagami K, Uramura K

机构信息

Department of Physiology, Faculty of Medicine, Kagoshima University, Japan.

出版信息

Neurosci Lett. 2001 Mar 9;300(2):91-4. doi: 10.1016/s0304-3940(01)01553-1.

DOI:10.1016/s0304-3940(01)01553-1

PMID:11207382

Abstract

Cholecystokinin-8 (CCK-8) dose-dependently increased the cytosolic Ca2+ concentration ([Ca]i) in ventromedial hypothalamic neurons acutely dissociated from the immature rat brain. The CCK-8 response was mimicked by caerulein, but not by CCK(B) agonists, and was often inhibited by CCK(A) receptor antagonists, but rarely by CCK(B) receptor antagonists. The response was dependent on external Ca2+ and Na+, and was inhibited by voltage-dependent Ca2+ channel blockers. The results suggest that CCK-8-induced depolarization via CCK(A) receptors increased Ca2+ influx through a voltage-dependent Ca2+ channel, which in turn increased [Ca]i.

摘要

胆囊收缩素-8（CCK-8）可使从新生大鼠脑部分离出的下丘脑腹内侧神经元的胞质钙离子浓度（[Ca]i）呈剂量依赖性增加。蛙皮素可模拟CCK-8的反应，但CCK(B)激动剂则不能，并且CCK-8的反应常被CCK(A)受体拮抗剂抑制，但很少被CCK(B)受体拮抗剂抑制。该反应依赖于细胞外钙离子和钠离子，并被电压依赖性钙离子通道阻滞剂抑制。结果表明，CCK-8通过CCK(A)受体诱导的去极化增加了通过电压依赖性钙离子通道的钙离子内流，进而增加了[Ca]i。